Separation of cyclic 3',5'-nucleoside monophosphates from other nucleotides on aluminum oxide columns. Application to the assay of adenyl cyclase and guanyl cyclase.

Adenine Nucleotides/isolation & purification Adenosine Triphosphate Adenylyl Cyclases/analysis Aluminum Animals Buffers Cattle Cell Membrane/enzymology Chromatography Chromatography, DEAE-Cellulose Cyclic AMP/isolation & purification Cyclic GMP/isolation & purification Enzymes/analysis Guanine Nucleotides/isolation & purification Hydrogen-Ion Concentration Kidney/cytology,enzymology Methods Nucleosides/isolation & purification Nucleotides/analysis,isolation & purification Nucleotidyltransferases/analysis Oxides Phosphorus Isotopes Purines/isolation & purification Pyrimidines/isolation & purification Rats Serum Albumin, Bovine Tritium

Adenine Nucleotides Buffers Enzymes Guanine Nucleotides Nucleosides Nucleotides Oxides Phosphorus Isotopes Purines Pyrimidines Tritium Serum Albumin, Bovine Adenosine Triphosphate Aluminum Cyclic AMP Nucleotidyltransferases Adenylyl Cyclases Cyclic GMP