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PMID: 17151069 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

RecA can stimulate the relaxation activity of topoisomerase I: Molecular basis of topoisomerase-mediated genome-wide transcriptional responses in Escherichia coli.

Nucleic acids research ·Vol. 35 ·No. 1 ·2007-00-00 ·Pages 79-86

Reckinger AR, Jeong KS, Khodursky AB, Hiasa H

Abstract

The superhelicity of the chromosome, which is controlled by DNA topoisomerases, modulates global gene expression. Investigations of transcriptional responses to the modulation of gyrase function have identified two types of topoisomerase-mediated transcriptional responses: (i) steady-state changes elicited by a mutation in gyrase, such as the D82G mutation in GyrA, and (ii) dynamic changes elicited by the inhibition of gyrase. We hypothesize that the steady-state effects are due to the changes in biochemical properties of gyrase, whereas the dynamic effects are due to an imbalance between supercoiling and relaxation activities, which appears to be influenced by the RecA activity. Herein, we present biochemical evidence for hypothesized mechanisms. GyrA D82G gyrase exhibits a reduced supercoiling activity. The RecA protein can influence the balance between supercoiling and relaxation activities either by interfering with the activity of DNA gyrase or by facilitating the relaxation reaction. RecA has no effect on the supercoiling activity of gyrase but stimulates the relaxation activity of topoisomerase I. This stimulation is specific and requires formation of an active RecA filament. These results suggest that the functional interaction between RecA and topoisomerase I is responsible for RecA-mediated modulation of the relaxation-dependent transcriptional activity of the Escherichia coli chromosome.

MeSH Terms
DNA Gyrase/genetics,metabolism DNA Topoisomerases, Type I/metabolism DNA, Superhelical/metabolism Escherichia coli/enzymology,genetics Escherichia coli Proteins/metabolism Genome, Bacterial Mutation Rec A Recombinases/metabolism Staphylococcus aureus/enzymology Transcription, Genetic
Chemicals
DNA, Superhelical Escherichia coli Proteins Rec A Recombinases DNA Topoisomerases, Type I DNA Gyrase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Reckinger Amy R
Department of Pharmacology, University of Minnesota Medical School-Twin Cities, Minneapolis, MN 55455, USA.
Jeong Kyeong Soo
Khodursky Arkady B
Hiasa Hiroshi
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkl981
Published
2007-00-00
Epub
2006-00-06
Pages
79-86
Language
English
Region
England
NLM ID
0411011
PMCID
PMC1761438
Subset
IM
Grants
NIGMS NIH HHS · R01 GM066098 · United States
NIGMS NIH HHS · GM59465 · United States
NIGMS NIH HHS · GM66098 · United States
External Links
PubMed source DOI: 10.1093/nar/gkl981 PMC Full Text View journal details
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