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PMID: 1706590 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Generation and characterization of the human immunodeficiency virus type 1 mutants.

Archives of virology ·Vol. 117 ·No. 1-2 ·1991-00-00 ·Pages 45-58

Adachi A, Ono N, Sakai H, Ogawa K, Shibata R, Kiyomasu T, Masuike H, Ueda S

Abstract

Mutations were introduced by recombinant DNA techniques into 9 genes of an infectious molecular clone of human immunodeficiency virus type 1. The 24 mutants generated were characterized biochemically and biologically by transfection and infection experiments. None of the mutants which have mutations in gag (p17, p24, and p15 regions), pol (protease, reverse transcriptase, and endonuclease domains), env (gp120 region), tat, or rev were infectious, whereas vif, vpr, vpu, some of env (gp41) and nef mutants could grow in human CD4+ cells to various degrees. Of the non-infectious mutants, only endonuclease (pol) and gp41 mutants exhibited normal phenotypes with respect to the production of functional reverse transcriptase, the expression of gag, pol, and env proteins, and the generation of progeny virions, when examined in transient assays. All infectious mutants killed the CD4+ cells with the exception of a mutant carrying a defect in the vif gene.

MeSH Terms
Blotting, Western DNA, Recombinant DNA, Viral Genes, Viral HIV-1/genetics,metabolism,pathogenicity,ultrastructure Mutagenesis Mutation/genetics RNA-Directed DNA Polymerase/metabolism Retroviridae Proteins/biosynthesis Transfection Virus Replication/genetics
Chemicals
DNA, Recombinant DNA, Viral Retroviridae Proteins RNA-Directed DNA Polymerase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Adachi A
Institute for Virus Research, Kyoto University, Japan.
Ono N
Sakai H
Ogawa K
Shibata R
Kiyomasu T
Masuike H
Ueda S
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Article Info
Journal
Archives of virology
Abbr.
Arch Virol
ISSN
0304-8608
DOI
10.1007/BF01310491
Published
1991-00-00
Pages
45-58
Language
English
Region
Austria
NLM ID
7506870
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01310491 View journal details
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