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PMID: 17021071 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Common virulence factors and genetic relationships between O18:K1:H7 Escherichia coli isolates of human and avian origin.

Journal of clinical microbiology ·Vol. 44 ·No. 10 ·2006-10-00 ·Pages 3484-92

Moulin-Schouleur M, Schouler C, Tailliez P, Kao MR, Brée A, Germon P, Oswald E, Mainil J, Blanco M, Blanco J

Abstract

Extraintestinal pathogenic (ExPEC) Escherichia coli strains of serotype O18:K1:H7 are mainly responsible for neonatal meningitis and sepsis in humans and belong to a limited number of closely related clones. The same serotype is also frequently isolated from the extraintestinal lesions of colibacillosis in poultry, but it is not well known to what extent human and avian strains of this particular serotype are related. Twenty-two ExPEC isolates of human origin and 33 isolates of avian origin were compared on the basis of their virulence determinants, lethality for chicks, pulsed-field gel electrophoresis (PFGE) patterns, and classification in the main phylogenetic groups. Both avian and human isolates were lethal for chicks and harbored similar virulence genotypes. A major virulence pattern, identified in 75% of the isolates, was characterized by the presence of F1 variant fimbriae; S fimbriae; IbeA; the aerobactin system; and genomic fragments A9, A12, D1, D7, D10, and D11 and by the absence of P fimbriae, F1C fimbriae, Afa adhesin, and CNF1. All but one of the avian and human isolates also belonged to major phylogenetic group B2. However, various subclonal populations could be distinguished by PFGE in relation to animal species and geographical origin. These results demonstrate that very closely related clones can be recovered from extraintestinal infections in humans and chickens and suggest that avian pathogenic E. coli isolates of serotype O18:K1:H7 are potential human pathogens.

MeSH Terms
Animals Chickens/microbiology Escherichia coli/classification,genetics,metabolism,pathogenicity Humans Phylogeny Virulence Virulence Factors/metabolism
Chemicals
Virulence Factors
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Moulin-Schouleur Maryvonne
INRA, Centre de Tours, UR1282 IASP, Pathogénie Bactérienne, 37380 Nouzilly, France.
Schouler Catherine
Tailliez Patrick
Kao Mu-Rong
Brée Annie
Germon Pierre
Oswald Eric
Mainil Jacques
Blanco Miguel
Blanco Jorge
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/JCM.00548-06
Published
2006-10-00
Pages
3484-92
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC1594794
Subset
IM
External Links
PubMed source DOI: 10.1128/JCM.00548-06 PMC Full Text View journal details
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