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PMID: 1701241 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Group II intron RNA-catalyzed recombination of RNA in vitro.

Nucleic acids research ·Vol. 18 ·No. 22 ·1990-11-25 ·Pages 6545-51

Mörl M, Schmelzer C

Abstract

We report the first evidence for a novel reaction mediated by the self-splicing yeast mitochondrial group II intron bl1; the site-specific recombination of RNA molecules in vitro. Upon incubation of the intron lariat with two different RNAs, each harbouring a short sequence complementary to exon binding site 1 (EBS1) of the intron, novel recombined RNAs are formed. As a result of this intron-mediated shuffling of gene segments, the 5' part of RNA1 is ligated to the 3' part of RNA2 and, reciprocally, the 5' part of RNA2 to the 3' part of RNA1. Sequence analysis of the recombinant junction shows that the site of recombination is precisely located 3' to intron binding site 1 (IBS1). The hypothesized mechanism of recombination involves exchange of RNA 5' parts after the first step of a reverse splicing reaction. The possible role of this mechanism in vivo and during prebiotic evolution is discussed.

MeSH Terms
Base Sequence Binding Sites Biological Evolution Exons Introns Molecular Sequence Data Nucleic Acid Conformation RNA/chemistry,metabolism RNA Splicing Recombination, Genetic Transcription, Genetic
Chemicals
RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mörl M
Institut für Genetik und Mikrobiologie, Universität München, FRG.
Schmelzer C
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34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.22.6545
Published
1990-11-25
Pages
6545-51
Language
English
Region
England
NLM ID
0411011
PMCID
PMC332608
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/18.22.6545 PMC Full Text View journal details
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