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PMID: 3412889 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Selection of cryptic 5' splice sites by group II intron RNAs in vitro.

Nucleic acids research ·Vol. 16 ·No. 15 ·1988-08-11 ·Pages 7383-95

Müller MW, Schweyen RJ, Schmelzer C

Abstract

Recognition of 5' splice points by group I and group II self-splicing introns involves the interaction of exon sequences--directly preceding the 5' splice site--with intronic sequence elements. We show here that the exon binding sequences (EBS) of group II intron aI5c can accept various substitutes of the authentic intron binding sites (IBS) provided in cis or in trans. The efficiency of cleavages at these cryptic 5' splice sites was enhanced by deletion of the authentic IBS2 element. All cryptic 5' cleavage sites studied here were preceded by an IBS1 like sequence; indicating that the IBS1/EBS1 pairing alone is sufficient for proper 5' splice site selection by the intronic EBS element. The results are discussed in terms of minimal requirements for 5' cleavages and position effects of IBS sites relative to the intron.

MeSH Terms
Exons Introns Nucleic Acid Precursors/metabolism Plasmids RNA Splicing RNA, Messenger/genetics Regulatory Sequences, Nucleic Acid Structure-Activity Relationship
Chemicals
Nucleic Acid Precursors RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Müller M W
Institut für Genetik und Mikrobiologie, Universität München, FRG.
Schweyen R J
Schmelzer C
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.15.7383
Published
1988-08-11
Pages
7383-95
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338415
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.15.7383 PMC Full Text View journal details
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