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PMID: 3004949 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Role of the 5' hairpin structure in the splicing accuracy of the fourth intron of the yeast cob-box gene.

The EMBO journal ·Vol. 4 ·No. 12 ·1985-12-01 ·Pages 3281-8

Perea J, Jacq C

Abstract

The splicing mechanism of the maturase-coding introns is poorly understood. We have systematically examined the phenotypes of a large number of revertants from the mitochondrial mutation G2457. This mutation results from a single base change near the 5' splicing site. We show here that this base change does not completely block the splicing of the intron but rather affects the specificity of the splicing process. We examine four classes of revertants which allow us to characterize the crucial role of a stem and loop structure in the accuracy of the intron excision process. An unexpected class of revertant suggests that other elements are involved in this mechanism. Reversion of G2457 can also occur via the excision in the mitochondrial genome of the intron coding sequence. These results are discussed in relation to the possible role fulfilled by the maturase in the control of intron splicing.

MeSH Terms
Animals Base Sequence DNA Restriction Enzymes DNA, Mitochondrial/genetics Endoribonucleases Genes Genes, Fungal Humans Mutation Nucleic Acid Conformation Nucleotidyltransferases/genetics Phenotype RNA Splicing Saccharomyces cerevisiae/genetics Species Specificity Yeasts/enzymology,genetics
Chemicals
DNA, Mitochondrial Nucleotidyltransferases mRNA maturase Endoribonucleases DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Perea J
Jacq C
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31 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-12-01
Pages
3281-8
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554655
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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