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PMID: 6262825 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Regulatory interactions between mitochondrial genes: interactions between two mosaic genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 3 ·1981-03-00 ·Pages 1778-82

Dhawale S, Hanson DK, Alexander NJ, Perlman PS, Mahler HR

Abstract

We have studied the mitochondrial DNA and the phenotypes of strains of Saccharomyces cerevisiae with specific intervening sequences in two mosaic genes: cob (the gene for apocytochrome b) and oxi3 (the gene for subunit I of cytochrome oxidase). The results suggest the following. (i) The presence of an intervening sequence downstream encompassing the intron box7 is sufficient for the regulation of oxi3 by cob (BOX phenotype); two sequences (containing intron loci box3 and box10) upstream in cob and two in oxi3 are dispensable. (ii) Strains without the two sequences upstream still contain the downstream sequence and the competence to specify a functional trans-acting element. Mutational lesions in this segment are phenotypically indistinguishable from box7 mutants, including the accumulation of polypeptides with homologous amino acid sequences. (iii) A catabolite-sensitive BOX phenotype, characteristic of mutants in the first exon, requires the simultaneous presence of an adjacent intervening sequence. A model is presented in which a hypothetical product specified by an intron (locus box7) of the cob gene controls the expression of a second mosaic gene (oxi3).

MeSH Terms
DNA Restriction Enzymes DNA, Mitochondrial/genetics Genes, Regulator Mutation Phenotype Protein Biosynthesis Saccharomyces cerevisiae/genetics Species Specificity
Chemicals
DNA, Mitochondrial DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Dhawale S
Hanson D K
Alexander N J
Perlman P S
Mahler H R
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.3.1778
Published
1981-03-00
Pages
1778-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319217
Subset
IM
Grants
NIGMS NIH HHS · GM 12228 · United States
NIGMS NIH HHS · GM 21896 · United States
PHS HHS · K06 05060 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.3.1778 PMC Full Text View journal details
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