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PMID: 16997874 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Single nanoparticle photothermal tracking (SNaPT) of 5-nm gold beads in live cells.

Biophysical journal ·Vol. 91 ·No. 12 ·2006-12-15 ·Pages 4598-604

Lasne D, Blab GA, Berciaud S, Heine M, Groc L, Choquet D, Cognet L, Lounis B

Abstract

Tracking individual nano-objects in live cells during arbitrary long times is a ubiquitous need in modern biology. We present here a method for tracking individual 5-nm gold nanoparticles on live cells. It relies on the photothermal effect and the detection of the Laser Induced Scattering around a NanoAbsorber (LISNA). The key point for recording trajectories at video rate is the use of a triangulation procedure. The effectiveness of the method is tested against single fluorescent molecule tracking in live COS7 cells on subsecond timescales. We further demonstrate recordings for several minutes of AMPA receptors trajectories on the plasma membrane of live neurons. Single Nanoparticle Photothermal Tracking has the unique potential to record arbitrary long trajectory of membrane proteins using nonfluorescent nanometer-sized labels.

MeSH Terms
Animals Biological Transport COS Cells Carbocyanines Cell Membrane/metabolism Cells, Cultured Chlorocebus aethiops Fluorescent Dyes Gold/chemistry Lasers Light Metal Nanoparticles Microscopy, Fluorescence Neurons/metabolism Receptors, Glutamate/metabolism Scattering, Radiation Temperature
Chemicals
Carbocyanines Fluorescent Dyes Receptors, Glutamate cyanine dye 5 Gold
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Lasne David
Centre de Physique Moléculaire Optique et Hertzienne, CNRS (UMR 5798) and Université Bordeaux 1, Talence Cedex, France.
Blab Gerhard A
Berciaud Stéphane
Heine Martin
Groc Laurent
Choquet Daniel
Cognet Laurent
Lounis Brahim
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1529/biophysj.106.089771
Published
2006-12-15
Epub
2006-00-22
Pages
4598-604
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1779909
Subset
IM
External Links
PubMed source DOI: 10.1529/biophysj.106.089771 PMC Full Text View journal details
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