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PMID: 13679586 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Single metallic nanoparticle imaging for protein detection in cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 100 ·No. 20 ·2003-09-30 ·Pages 11350-5

Cognet L, Tardin C, Boyer D, Choquet D, Tamarat P, Lounis B

Abstract

We performed a visualization of membrane proteins labeled with 10-nm gold nanoparticles in cells, using an all-optical method based on photothermal interference contrast. The high sensitivity of the method and the stability of the signals allows 3D imaging of individual nanoparticles without the drawbacks of photobleaching and blinking inherent to fluorescent markers. A simple analytical model is derived to account for the measurements of the signal amplitude and the spatial resolution. The photothermal interference contrast method provides an efficient, reproducible, and promising way to visualize low amounts of proteins in cells by optical means.

MeSH Terms
Animals COS Cells Fluorescence Immunohistochemistry Metals/chemistry Particle Size Proteins/analysis Scattering, Radiation
Chemicals
Metals Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cognet L
Centre de Physique Moléculaire Optique et Hertzienne, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5798 et Université Bordeaux 1, 351 Cours de la Libération, 33405 Talence, France.
Tardin C
Boyer D
Choquet D
Tamarat P
Lounis B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.1534635100
Published
2003-09-30
Epub
2003-00-17
Pages
11350-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC208760
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.1534635100 PMC Full Text View journal details
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