Home LiteratureArticle Details
PMID: 3427186 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nanovid tracking: a new automatic method for the study of mobility in living cells based on colloidal gold and video microscopy.

Biophysical journal ·Vol. 52 ·No. 5 ·1987-11-00 ·Pages 775-82

Geerts H, De Brabander M, Nuydens R, Geuens S, Moeremans M, De Mey J, Hollenbeck P

Abstract

We describe a new automatic technique for the study of intracellular mobility. It is based on the visualization of colloidal gold particles by video-enhanced contrast light microscopy (nanometer video microscopy) combined with modern tracking algorithms and image processing hardware. The approach can be used for determining the complete statistics of saltatory motility of a large number of individual moving markers. Complete distributions of jump time, jump velocity, stop time, and orientation can be generated. We also show that this method allows one to study the characteristics of random motion in the cytoplasm of living cells or on cell membranes. The concept is illustrated by two studies. First we present the motility of colloidal gold in an in vitro system of microtubules and a protein extract containing a kinesin-like factor. The algorithm is thoroughly tested by manual tracking of the videotapes. The second study involves the motion of gold particles microinjected in the cytoplasm of PTK-2 cells. Here the results are compared to a study using the spreading of colloidal gold particles after microinjection.

MeSH Terms
Animals Autoanalysis Cell Line Cell Movement Gold Microscopy/methods Video Recording
Chemicals
Gold
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Geerts H
Department of Life Sciences, Janssen Pharmaceutica Research Laboratories, Beerse, Belgium.
De Brabander M
Nuydens R
Geuens S
Moeremans M
De Mey J
Hollenbeck P
References (21)
21 references, click to expand
  1. Gliding movement of and bidirectional transport along single native microtubules from squid axoplasm: evidence for an active role of microtubules in cytoplasmic transport.
    J Cell Biol. 1985 May;100(5):1736-52 PMID: 2580845
  2. Nucleo-cytoplasmic flux and intracellular mobility in single hepatocytes measured by fluorescence microphotolysis.
    EMBO J. 1984 Aug;3(8):1831-6 PMID: 6207019
  3. Fluorescence correlation spectroscopy. II. An experimental realization.
    Biopolymers. 1974 Jan;13(1):29-61 PMID: 4818131
  4. The use of submicroscopic gold particles combined with video contrast enhancement as a simple molecular probe for the living cell.
    Cell Motil Cytoskeleton. 1986;6(2):105-13 PMID: 3011284
  5. Improved system for capillary microinjection into living cells.
    Exp Cell Res. 1982 Jul;140(1):31-7 PMID: 6286332
  6. A model for the diffusion of fluorescent probes in the septate giant axon of earthworm. Axoplasmic diffusion and junctional membrane permeability.
    Biophys J. 1985 Aug;48(2):299-309 PMID: 4052564
  7. Video-enhanced contrast polarization (AVEC-POL) microscopy: a new method applied to the detection of birefringence in the motile reticulopodial network of Allogromia laticollaris.
    Cell Motil. 1981;1(3):275-89 PMID: 7348604
  8. Probing microtubule-dependent intracellular motility with nanometre particle video ultramicroscopy (nanovid ultramicroscopy).
    Cytobios. 1985;43(174S):273-83 PMID: 3907999
  9. The translational mobility of substances within the cytoplasmic matrix.
    Proc Natl Acad Sci U S A. 1984 Nov;81(21):6747-51 PMID: 6387711
  10. Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.
    Biophys J. 1976 Sep;16(9):1055-69 PMID: 786399
  11. Identification of a novel force-generating protein, kinesin, involved in microtubule-based motility.
    Cell. 1985 Aug;42(1):39-50 PMID: 3926325
  12. Anisotropic molecular motion on cell surfaces.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5641-4 PMID: 293666
  13. Diffusion of low density lipoprotein-receptor complex on human fibroblasts.
    J Cell Biol. 1982 Dec;95(3):846-52 PMID: 6296157
  14. The cytoplasmic matrix: its volume and surface area and the diffusion of molecules through it.
    Proc Natl Acad Sci U S A. 1985 Aug;82(15):5030-4 PMID: 3860842
  15. Rates of membrane-associated reactions: reduction of dimensionality revisited.
    J Cell Biol. 1986 Jan;102(1):88-96 PMID: 3001105
  16. Diffusion of injected macromolecules within the cytoplasm of living cells.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4407-10 PMID: 6945591
  17. Rates of diffusion controlled reactions in one, two and three dimensions.
    Biophys Chem. 1979 Nov;10(3-4):239-43 PMID: 16997220
  18. The association of a class of saltatory movements with microtubules in cultured cells.
    J Cell Biol. 1970 May;45(2):334-54 PMID: 5513607
  19. Motion of mitochondria in cultured cells quantified by analysis of digitized images.
    Biophys J. 1985 Nov;48(5):681-6 PMID: 4074829
  20. Association of high-molecular-weight proteins with microtubules and their role in microtubule assembly in vitro.
    Proc Natl Acad Sci U S A. 1975 Jul;72(7):2696-700 PMID: 1058484
  21. Direct measurement of lateral transport in membranes by using time-resolved spatial photometry.
    Proc Natl Acad Sci U S A. 1985 Jun;82(12):4122-6 PMID: 3858869
Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(87)83271-X
Published
1987-11-00
Pages
775-82
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1330181
Subset
IM
External Links
PubMed source DOI: 10.1016/S0006-3495(87)83271-X PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2