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PMID: 1698619 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Colony stimulating factor-1 (CSF-1) stimulates temperature dependent phosphorylation and activation of the RAF-1 proto-oncogene product.

The EMBO journal ·Vol. 9 ·No. 11 ·1990-11-00 ·Pages 3649-57

Baccarini M, Sabatini DM, App H, Rapp UR, Stanley ER

Abstract

The serine/threonine kinase RAF-1 is phosphorylated in intact macrophages in response to CSF-1 at 37 degrees C The augmented phosphorylation of RAF-1 and a concomitant increase in RAF-1 associated serine/threonine kinase activity are kinetically later events than CSF-1 induced protein tyrosine phosphorylation. Furthermore, phosphoamino acid analysis of RAF-1 reveals the presence of phosphoserine, trace amounts of phosphothreonine but no phosphotyrosine and the phosphorylated RAF-1 does not react with anti-phosphotyrosine antibodies. In contrast to CSF-1 induced protein tyrosine phosphorylation, RAF-1 phosphorylation and activation are temperature dependent and do not occur at 4 degrees C. Furthermore, coprecipitation experiments failed to reveal any noncovalent association of RAF-1 with the CSF-1 receptor. Thus, while RAF-1 is not a direct substrate for the CSF-1 receptor tyrosine kinase in vivo, its temperature dependent phosphorylation and activation represent an intriguing aspect of the CSF-1 response.

MeSH Terms
Amino Acid Sequence Animals In Vitro Techniques Macrophage Colony-Stimulating Factor/pharmacology Macrophages/metabolism Mice Molecular Sequence Data Oligopeptides/metabolism Phosphorylation Phosphoserine/metabolism Phosphothreonine/metabolism Phosphotyrosine Platelet-Derived Growth Factor/pharmacology Precipitin Tests Protein Kinases/metabolism Protein Serine-Threonine Kinases Proto-Oncogene Proteins/metabolism Proto-Oncogene Proteins c-raf Receptor, Macrophage Colony-Stimulating Factor/metabolism Receptors, Cell Surface/metabolism Receptors, Platelet-Derived Growth Factor Temperature Tyrosine/analogs & derivatives,metabolism
Chemicals
Oligopeptides Platelet-Derived Growth Factor Proto-Oncogene Proteins Receptors, Cell Surface Phosphothreonine Phosphoserine Phosphotyrosine Tyrosine Macrophage Colony-Stimulating Factor Protein Kinases Receptor, Macrophage Colony-Stimulating Factor Receptors, Platelet-Derived Growth Factor Protein Serine-Threonine Kinases Proto-Oncogene Proteins c-raf
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Baccarini M
Department of Developmental Biology and Cancer, Albert Einstein College of Medicine, Bronx, NY 10461.
Sabatini D M
App H
Rapp U R
Stanley E R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-11-00
Pages
3649-57
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552119
Subset
IM
Grants
NCI NIH HHS · CA 26504 · United States
NCI NIH HHS · CA 32551 · United States
NCI NIH HHS · P30-CA1330 · United States
External Links
PubMed source PMC Full Text View journal details
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