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PMID: 2837654 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ligand-induced tyrosine kinase activity of the colony-stimulating factor 1 receptor in a murine macrophage cell line.

Molecular and cellular biology ·Vol. 8 ·No. 4 ·1988-04-00 ·Pages 1795-9

Downing JR, Rettenmier CW, Sherr CJ

Abstract

Metabolic labeling of simian virus 40-immortalized murine macrophages with 32Pi and immunoblotting with antibodies to phosphotyrosine demonstrated that the c-fms proto-oncogene product (colony-stimulating factor 1 [CSF-1] receptor) was phosphorylated on tyrosine in vivo and rapidly degraded in response to CSF-1. Stimulation of the CSF-1 receptor also induced immediate phosphorylation of several other cellular proteins on tyrosine. By contrast, the mature cell surface glycoprotein encoded by the v-fms oncogene was phosphorylated on tyrosine in the absence of CSF-1, suggesting that it functions as a ligand-independent kinase.

MeSH Terms
Animals Cell Line Colony-Stimulating Factors/metabolism Kinetics Ligands Macrophages/metabolism Mice Phosphorus Radioisotopes Phosphorylation Protein-Tyrosine Kinases/metabolism Proto-Oncogenes Receptors, Cell Surface/metabolism Receptors, Colony-Stimulating Factor
Chemicals
Colony-Stimulating Factors Ligands Phosphorus Radioisotopes Receptors, Cell Surface Receptors, Colony-Stimulating Factor Protein-Tyrosine Kinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Downing J R
Department of Tumor Cell Biology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105.
Rettenmier C W
Sherr C J
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33 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.4.1795-1799.1988
Published
1988-04-00
Pages
1795-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363340
Subset
IM
Grants
NCI NIH HHS · CA38187 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.4.1795-1799.1988 PMC Full Text View journal details
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