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PMID: 3029775 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Purification of the colony-stimulating factor 1 receptor and demonstration of its tyrosine kinase activity.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 5 ·1987-03-00 ·Pages 1268-71

Yeung YG, Jubinsky PT, Sengupta A, Yeung DC, Stanley ER

Abstract

Colony-stimulating factor 1 (CSF-1) regulates the survival, proliferation, and differentiation of mononuclear phagocytes. The CSF-1 receptor was purified from cell membranes of the J774.2 mouse macrophage cell line by solubilization with Triton X-100, CSF-1 affinity chromatography, and gel filtration. The purified receptor is a protein or glycoprotein of 165 kDa comprising a single polypeptide chain that is not covalently associated, either as a homopolymer, or with any other protein. CSF-1 stimulated autophosphorylation of the purified receptor in tyrosine residues. Casein but not histone was shown to act as a substrate for the tyrosine protein kinase activity of purified receptor.

MeSH Terms
Animals Cell Line Colony-Stimulating Factors/metabolism Kinetics Macrophages Mice Molecular Weight Phosphorylation Protein-Tyrosine Kinases/metabolism Receptors, Cell Surface/isolation & purification,metabolism Receptors, Colony-Stimulating Factor
Chemicals
Colony-Stimulating Factors Receptors, Cell Surface Receptors, Colony-Stimulating Factor Protein-Tyrosine Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Yeung Y G
Jubinsky P T
Sengupta A
Yeung D C
Stanley E R
References (25)
25 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. Uptake and destruction of 125I-CSF-1 by peritoneal exudate macrophages.
    J Cell Biochem. 1986;31(3):203-16 PMID: 2978971
  3. A simple method for the isolation of the subcomponents of the first component of complement by affinity chromatography.
    J Immunol. 1974 Jul;113(1):225-34 PMID: 4857625
  4. Reticulum cell sarcoma: an effector cell in antibody-dependent cell-mediated immunity.
    J Immunol. 1975 Feb;114(2 pt 2):898-905 PMID: 1089721
  5. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  6. Factors regulating macrophage production and growth. Purification and some properties of the colony stimulating factor from medium conditioned by mouse L cells.
    J Biol Chem. 1977 Jun 25;252(12):4305-12 PMID: 301140
  7. The Fc receptors of primary and cultured phagocytic cells studied with homogeneous antibodies.
    J Immunol. 1978 Oct;121(4):1329-33 PMID: 701797
  8. Specific interaction of murine colony-stimulating factor with mononuclear phagocytic cells.
    J Cell Biol. 1980 Apr;85(1):153-9 PMID: 6965942
  9. Identification of phosphotyrosine as a product of epidermal growth factor-activated protein kinase in A-431 cell membranes.
    J Biol Chem. 1980 Sep 25;255(18):8363-5 PMID: 6157683
  10. Methods for the purification, assay, characterization and target cell binding of a colony stimulating factor (CSF-1).
    J Immunol Methods. 1981;42(3):253-84 PMID: 6974750
  11. Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.
    Anal Biochem. 1981 Nov 1;117(2):307-10 PMID: 6172996
  12. Distribution of cells bearing receptors for a colony-stimulating factor (CSF-1) in murine tissues.
    J Cell Biol. 1981 Dec;91(3 Pt 1):848-53 PMID: 6276411
  13. Identification and characterization of a latent pool of insulin receptors in 3T3-L1 adipocytes.
    J Biol Chem. 1982 May 25;257(10):5350-8 PMID: 7068595
  14. Purification and characterization of epidermal growth factor receptor/protein kinase from normal mouse liver.
    Proc Natl Acad Sci U S A. 1982 Oct;79(20):6237-41 PMID: 6983068
  15. Characterization of the receptor for platelet-derived growth factor on human fibroblasts. Demonstration of an intimate relationship with a 185,000-Dalton substrate for the platelet-derived growth factor-stimulated kinase.
    J Biol Chem. 1983 Aug 25;258(16):10054-61 PMID: 6309764
  16. CSF-1--a mononuclear phagocyte lineage-specific hemopoietic growth factor.
    J Cell Biochem. 1983;21(2):151-9 PMID: 6309875
  17. Chemical crosslinking of the mononuclear phagocyte specific growth factor CSF-1 to its receptor at the cell surface.
    Biochem Biophys Res Commun. 1984 Feb 29;119(1):35-41 PMID: 6322783
  18. Factors affecting the growth and differentiation of haemopoietic cells in culture.
    Clin Haematol. 1984 Jun;13(2):329-48 PMID: 6088143
  19. Specific expression of the human cellular fps/fes-encoded protein NCP92 in normal and leukemic myeloid cells.
    Proc Natl Acad Sci U S A. 1985 Apr;82(8):2379-83 PMID: 2986115
  20. The c-fms proto-oncogene product is related to the receptor for the mononuclear phagocyte growth factor, CSF-1.
    Cell. 1985 Jul;41(3):665-76 PMID: 2408759
  21. The interaction of 125I-colony-stimulating factor-1 with bone marrow-derived macrophages.
    J Biol Chem. 1986 Mar 25;261(9):4024-32 PMID: 3485098
  22. Specific binding of the mononuclear phagocyte colony-stimulating factor CSF-1 to the product of the v-fms oncogene.
    Proc Natl Acad Sci U S A. 1986 May;83(10):3331-5 PMID: 3010289
  23. Expression of the human c-fms proto-oncogene product (colony-stimulating factor-1 receptor) on peripheral blood mononuclear cells and choriocarcinoma cell lines.
    J Clin Invest. 1986 Jun;77(6):1740-6 PMID: 3011859
  24. Solubilization and assay of a colony-stimulating factor receptor from murine macrophages.
    J Cell Biochem. 1986;31(4):259-69 PMID: 3020066
  25. Isolation of the insulin receptor of liver and fat-cell membranes (detergent-solubilized-( 125 I)insulin-polyethylene glycol precipitation-sephadex).
    Proc Natl Acad Sci U S A. 1972 Feb;69(2):318-22 PMID: 4501116
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.5.1268
Published
1987-03-00
Pages
1268-71
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304408
Subset
IM
Grants
NCI NIH HHS · CA26504 · United States
NIGMS NIH HHS · GM7288 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.5.1268 PMC Full Text View journal details
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