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PMID: 1698277 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The organization and evolution of transfer RNA genes in Mycoplasma capricolum.

Nucleic acids research ·Vol. 18 ·No. 17 ·1990-09-11 ·Pages 5037-43

Muto A, Andachi Y, Yuzawa H, Yamao F, Osawa S

Abstract

The genes for presumably all the tRNA species in Mycoplasma capricolum, a derivative of Gram-positive eubacteria, have been cloned and sequenced. There are 30 genes encoding 29 tRNA species. This number is the smallest in all the known genetic systems except for mitochondria. The sequences of 9 tRNA genes of them have been previously reported (1-3). Twenty-two genes are organized in 5 clusters consisting of nine, five, four and two genes (2 sets), respectively. The other eight genes exist as a single transcription unit. All the tRNAs are encoded each by a single gene, except for the occurrence of two tRNA(Lys)(TTT) genes. The arrangement of tRNA genes in the 9-gene cluster, the 5-gene cluster, the 4-gene cluster and one of the 2-gene clusters reveals extensive similarity with a part of the 21-tRNA gene cluster and/or the 16-tRNA gene cluster in Bacillus subtilis, respectively. The results suggest that the present M. capricolum tRNA genes have evolved from large tRNA gene clusters in the ancestral Gram-positive bacterial genome common to M. capricolum and B. subtilis, by discarding genes for redundant as well as non-obligate tRNAs, so that all the codons may be translated by as small a number of tRNAs as possible.

MeSH Terms
Base Sequence Biological Evolution Cloning, Molecular Genes, Bacterial Molecular Sequence Data Multigene Family Mycoplasma/genetics RNA, Bacterial/genetics RNA, Transfer/genetics Sequence Homology, Nucleic Acid
Chemicals
RNA, Bacterial RNA, Transfer
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Muto A
Department of Biology, School of Science, Nagoya University, Japan.
Andachi Y
Yuzawa H
Yamao F
Osawa S
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34 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/18.17.5037
Published
1990-09-11
Pages
5037-43
Language
English
Region
England
NLM ID
0411011
PMCID
PMC332117
Subset
IM
Databases
GENBANK
D00547, D00548, D00549, D00550, D00551, D00552, D00553, D00554, D00555, D00556
External Links
PubMed source DOI: 10.1093/nar/18.17.5037 PMC Full Text View journal details
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