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PMID: 6222284 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The nucleotide sequence of Bacillus subtilis tRNA genes.

Nucleic acids research ·Vol. 11 ·No. 10 ·1983-05-25 ·Pages 3037-45

Yamada Y, Ohki M, Ishikura H

Abstract

Clones carring Bacillus subtilis tRNA genes were isolated from a lambda 816 library. A recombinant phage lambda 816-BS83 which was hybridized effectively with unfractionated tRNA probes contained a 3-kb fragment. By a Southern's blot analysis, it was found that tRNA genes were located in Eco RI-Hinc II region of this fragment. Sequence determination revealed the presence of a cluster of four tRNA genes in this region. The gene organization was as follows: tDNALys-9bp-tDNAGlu-81bp-tDNAAsp-30bp-tDNAPhe. The RNA sequences expected from tDNALys and tDNAPhe were identical with the reported RNA sequences. Two tRNA genes, tDNALys and tDNAAsp encoded the CCA sequence of 3'-terminal region, but the other two, tDNAGlu and tDNAPhe did not. A promoter-like sequence which corresponds to the sigma 55-recognition site was found in a region about 100bp upstream from tDNALys.

MeSH Terms
Bacillus subtilis/genetics Bacteriophage lambda/genetics Base Sequence Cloning, Molecular Genes Genes, Bacterial Nucleic Acid Conformation Nucleic Acid Hybridization RNA, Transfer/genetics Transcription, Genetic
Chemicals
RNA, Transfer
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Yamada Y
Ohki M
Ishikura H
References (11)
11 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.10.3037
Published
1983-05-25
Pages
3037-45
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325946
Subset
IM
Databases
GENBANK
K00968
External Links
PubMed source DOI: 10.1093/nar/11.10.3037 PMC Full Text View journal details
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