A potato mitochondrial isoleucine tRNA is coded for by a mitochondrial gene possessing a methionine anticodon.

Nucleic acids research ·Vol. 18 ·No. 17 ·1990-09-11 ·Pages 5027-30

Weber F, Dietrich A, Weil JH, Maréchal-Drouard L

Abstract

The nucleotide sequence of a potato mitochondrial tRNA(Ile) has been determined. This tRNA has a lysidine-like minor nucleotide in the first position of the anticodon (position 34). The corresponding mitochondrial gene has been cloned and sequenced. This gene has a CAT anticodon which corresponds to methionine. The C residue in the anticodon must therefore be post-transcriptionally modified. The mature tRNA has isoleucine-accepting activity but no methionine-accepting activity. This is the first report showing that in plant mitochondria a post-transcriptional modification could change the amino-acid specificity of a tRNA, as compared to that of the corresponding gene.

MeSH Terms

Anticodon Base Sequence Blotting, Southern Chromatography, Thin Layer Cloning, Molecular DNA, Mitochondrial/genetics Molecular Sequence Data Nucleic Acid Conformation RNA/genetics RNA Processing, Post-Transcriptional RNA, Mitochondrial RNA, Transfer, Amino Acid-Specific/genetics RNA, Transfer, Ile/genetics,metabolism RNA, Transfer, Met/genetics Sequence Homology, Nucleic Acid Solanum tuberosum/genetics

Chemicals

Anticodon DNA, Mitochondrial RNA, Mitochondrial RNA, Transfer, Amino Acid-Specific RNA, Transfer, Ile RNA, Transfer, Met RNA

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Weber F

Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, Strasbourg, France.

Dietrich A

Weil J H

Maréchal-Drouard L

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/18.17.5027

Published

1990-09-11

Pages

5027-30

Language

English

Region

England

NLM ID

0411011

PMCID

PMC332112

Subset

Databases

GENBANK

X53391, X53392

External Links

PubMed source DOI: 10.1093/nar/18.17.5027 PMC Full Text View journal details

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A potato mitochondrial isoleucine tRNA is coded for by a mitochondrial gene possessing a methionine anticodon.

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