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PMID: 2762145 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Chloroplast-like transfer RNA genes expressed in wheat mitochondria.

Nucleic acids research ·Vol. 17 ·No. 14 ·1989-07-25 ·Pages 5461-76

Joyce PB, Gray MW

Abstract

In the course of a systematic survey of wheat mitochondrial tRNA genes, we have sequenced chloroplast-like serine (trnS-GGA), phenylalanine (trnF-GAA) and cysteine (trnC-GCA) tRNA genes and their flanking regions. These genes are remnants of 'promiscuous' chloroplast DNA that has been incorporated into wheat mtDNA in the course of its evolution. Each gene differs by one or a few nucleotides from the authentic chloroplast homolog previously characterized in wheat or other plants, and each could potentially encode a functional tRNA whose secondary structure shows no deviations from the generalized model. To determine whether these chloroplast-like tRNA genes are actually expressed, wheat mitochondrial tRNAs were resolved by a series of polyacrylamide gel electrophoreses, after being specifically end-labeled in vitro by 3'-CCA addition mediated by wheat tRNA nucleotidyltransferase. Subsequent direct RNA sequence analysis identified prominent tRNA species corresponding to the mitochondrial and not the chloroplast trnS, trnF and trnC genes. This analysis also revealed chloroplast-like elongator methionine, asparagine and tryptophan tRNAs. Our results suggest that at least some chloroplast-like tRNA genes in wheat mtDNA are transcribed, with transcripts undergoing processing, post-transcriptional modification and 3'-CCA addition, to produce mature tRNAs that may participate in mitochondrial protein synthesis.

MeSH Terms
Base Sequence Chloroplasts/metabolism Cloning, Molecular DNA, Mitochondrial/genetics,isolation & purification Genes Mitochondria/metabolism Molecular Sequence Data Nucleic Acid Conformation Plants/genetics,metabolism Plasmids RNA, Transfer/genetics Triticum/genetics,metabolism
Chemicals
DNA, Mitochondrial RNA, Transfer
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Joyce P B
Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia, Canada.
Gray M W
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.14.5461
Published
1989-07-25
Pages
5461-76
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318170
Subset
IM
Databases
GENBANK
X15118, X15119
External Links
PubMed source DOI: 10.1093/nar/17.14.5461 PMC Full Text View journal details
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