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PMID: 16941221 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Heterosis and polymorphisms of gene expression in an elite rice hybrid as revealed by a microarray analysis of 9198 unique ESTs.

Plant molecular biology ·Vol. 62 ·No. 4-5 ·2006-11-00 ·Pages 579-91

Huang Y, Zhang L, Zhang J, Yuan D, Xu C, Li X, Zhou D, Wang S, Zhang Q

Abstract

Despite the significant contributions of utilizing heterosis to crop productivity worldwide, the biological mechanisms of heterosis remained largely uncharacterized. In this study, we analyzed gene expression profiles of an elite rice hybrid and the parents at three stages of young panicle development, using a cDNA microarray consisting of 9198 expressed sequence tags (ESTs), with the objective to reveal patterns of gene expression that may be associated with heterosis in yield. A total of 8422 sequences showed hybridization signals in all three genotypes in at least one stage and 5771 sequences produced detectable signals in all slides. Significant differences in expression level were detected for 438 sequences among the three genotypes in at least one of the three stages, as determined by ANOVA validated with 100 permutations at P < 0.05. Significant mid-parent heterosis was detected for 141 sequences, which demonstrated the following features: a much larger number of sequences showed negative heterosis than ones showing positive heterosis; genes functioning in DNA replication and repair tended to show positive heterosis; genes functioning in carbohydrate metabolism, lipid metabolism, energy metabolism, translation, protein degradation, and cellular information processing showed negative heterosis; both positive and negative heterosis were observed for genes in amino acid metabolism, transcription, signal transduction, plant defense and transportation. The results are indicative of the biochemical and physiological activities taking place in the hybrid relative to the parents. Identification of genes showing expression polymorphisms among different genotypes and heterotic expression in the hybrid may provide new avenues for exploring the biological mechanisms underlying heterosis.

MeSH Terms
Analysis of Variance DNA, Complementary Expressed Sequence Tags Genotype Oligonucleotide Array Sequence Analysis Oryza/genetics Polymorphism, Genetic
Chemicals
DNA, Complementary
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Huang Yi
National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research Wuhan, Huazhong Agricultural University, Wuhan 430070, China.
Zhang Lida
Zhang Jianwei
Yuan Dejun
Xu Caiguo
Li Xianghua
Zhou Daoxiu
Wang Shiping
Zhang Qifa
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/s11103-006-9040-z
Published
2006-11-00
Epub
2006-00-29
Pages
579-91
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/s11103-006-9040-z View journal details
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