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PMID: 16514558 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

The early dark-response in Arabidopsis thaliana revealed by cDNA microarray analysis.

Plant molecular biology ·Vol. 60 ·No. 3 ·2006-02-00 ·Pages 321-42

Kim BH, von Arnim AG

Abstract

Despite intense research on light responses in plants, the consequences of a simple shift from light to darkness remain poorly characterized. We have examined the transcriptome of Arabidopsis thaliana seedling leaves upon a shift from constant light to darkness for between 1 and 8 h, while excluding most effects associated with circadian oscillation. Expression clustering and gene ontology analyses identified about 790 responsive genes implicated in diverse cellular processes. Compared to the better-studied long-term dark adaptation response, the early response to darkness is partially overlapping yet clearly distinct, encompassing early transient, early sustained, and late response clusters. The repressor of photomorphogenesis, COP1 (constitutive photomorphogenic 1), is not a chief regulator of the early response to darkness, in contrast to its well-established role during long-term dark adaptation and etiolation. Only part of the early dark response can be understood as the opposite of the response following a dark-to-light transition and as a response to sugar deprivation. Bioinformatic comparisons with published microarray datasets further suggest that abscisic acid (ABA) signaling plays a prominent role in the early response to darkness, although this effect is not mediated by an increase in the ABA level. The potential basis for the co-regulation by darkness and ABA is discussed in light of sugar and redox signaling.

MeSH Terms
Abscisic Acid/metabolism Arabidopsis/growth & development,metabolism,physiology Arabidopsis Proteins/metabolism Blotting, Northern Carbohydrates Circadian Rhythm Cluster Analysis Computational Biology/methods DNA, Complementary/metabolism Darkness Down-Regulation Gene Expression Regulation, Plant Genetic Techniques Glucose/pharmacology Golgi Apparatus/metabolism Light Microarray Analysis Models, Biological Mutation Oligonucleotide Array Sequence Analysis/methods Oscillometry Oxidation-Reduction Plant Physiological Phenomena RNA, Messenger/metabolism Regulatory Elements, Transcriptional Reverse Transcriptase Polymerase Chain Reaction Scattering, Radiation Signal Transduction Time Factors Up-Regulation
Chemicals
Arabidopsis Proteins Carbohydrates DNA, Complementary RNA, Messenger Abscisic Acid Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kim Byung-Hoon
Department of Biochemistry, Cellular and Molecular Biology, The University of Tennessee, Knoxville, TN 37996-0840, USA.
von Arnim Albrecht G
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/s11103-005-4211-x
Published
2006-02-00
Pages
321-42
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/s11103-005-4211-x View journal details
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