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PMID: 12837949 Published · ppublish English Journal Article

Gibberellin biosynthesis and response during Arabidopsis seed germination.

The Plant cell ·Vol. 15 ·No. 7 ·2003-07-00 ·Pages 1591-604

Ogawa M, Hanada A, Yamauchi Y, Kuwahara A, Kamiya Y, Yamaguchi S

Abstract

The hormone-mediated control of plant growth and development involves both synthesis and response. Previous studies have shown that gibberellin (GA) plays an essential role in Arabidopsis seed germination. To learn how GA stimulates seed germination, we performed comprehensive analyses of GA biosynthesis and response using gas chromatography-mass spectrometry and oligonucleotide-based DNA microarray analysis. In addition, spatial correlations between GA biosynthesis and response were assessed by in situ hybridization. We identified a number of transcripts, the abundance of which is modulated upon exposure to exogenous GA. A subset of these GA-regulated genes was expressed in accordance with an increase in endogenous active GA levels, which occurs just before radicle emergence. The GA-responsive genes identified include those responsible for synthesis, transport, and signaling of other hormones, suggesting the presence of uncharacterized crosstalk between GA and other hormones. In situ hybridization analysis demonstrated that the expression of GA-responsive genes is not restricted to the predicted site of GA biosynthesis, suggesting that GA itself, or GA signals, is transmitted across different cell types during Arabidopsis seed germination.

MeSH Terms
Abscisic Acid/pharmacology Arabidopsis/genetics,growth & development,metabolism Cell Division/drug effects Ethylenes/pharmacology Gas Chromatography-Mass Spectrometry Gene Expression Regulation, Developmental/drug effects Gene Expression Regulation, Plant/drug effects Germination/drug effects,genetics Gibberellins/biosynthesis,pharmacology In Situ Hybridization Indoleacetic Acids/pharmacology Multigene Family/genetics Mutation Oligonucleotide Array Sequence Analysis Plant Growth Regulators/pharmacology Seeds/genetics,growth & development,metabolism Signal Transduction/drug effects Transcription Factors/genetics
Chemicals
Ethylenes Gibberellins Indoleacetic Acids Plant Growth Regulators Transcription Factors Abscisic Acid ethylene gibberellic acid
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ogawa Mikihiro
Plant Science Center, RIKEN, Suehiro-cho 1-7-22, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan.
Hanada Atsushi
Yamauchi Yukika
Kuwahara Ayuko
Kamiya Yuji
Yamaguchi Shinjiro
References (75)
75 references, click to expand
  1. Polar auxin transport: controlling where and how much.
    Trends Plant Sci. 2001 Nov;6(11):535-42 PMID: 11701382
  2. Arabidopsis ABI5 subfamily members have distinct DNA-binding and transcriptional activities.
    Plant Physiol. 2002 Oct;130(2):688-97 PMID: 12376636
  3. Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.
    Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7276-80 PMID: 1871133
  4. The isolation of abscisic acid (ABA) deficient mutants by selection of induced revertants in non-germinating gibberellin sensitive lines of Arabidopsis thaliana (L.) heynh.
    Theor Appl Genet. 1982 Dec;61(4):385-93 PMID: 24270501
  5. Microarray analysis of developing Arabidopsis seeds.
    Plant Physiol. 2000 Dec;124(4):1570-81 PMID: 11115875
  6. Cluster analysis and display of genome-wide expression patterns.
    Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14863-8 PMID: 9843981
  7. GIBBERELLIN BIOSYNTHESIS: Enzymes, Genes and Their Regulation.
    Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:431-460 PMID: 15012270
  8. Transactivation of the Brassica napus napin promoter by ABI3 requires interaction of the conserved B2 and B3 domains of ABI3 with different cis-elements: B2 mediates activation through an ABRE, whereas B3 interacts with an RY/G-box.
    Plant J. 2000 Oct;24(1):57-66 PMID: 11029704
  9. Integration of floral inductive signals in Arabidopsis.
    Nature. 2000 Apr 20;404(6780):889-92 PMID: 10786797
  10. Abscisic acid, phaseic acid and gibberellin contents associated with dormancy and germination in barley.
    Physiol Plant. 2002 Jul;115(3):428-441 PMID: 12081536
  11. Gibberellins: perception, transduction and responses.
    Plant Mol Biol. 1994 Dec;26(5):1529-55 PMID: 7858203
  12. EIN4 and ERS2 are members of the putative ethylene receptor gene family in Arabidopsis.
    Plant Cell. 1998 Aug;10(8):1321-32 PMID: 9707532
  13. Abscisic acid signaling in seeds and seedlings.
    Plant Cell. 2002;14 Suppl:S15-45 PMID: 12045268
  14. Gibberellin metabolism: new insights revealed by the genes.
    Trends Plant Sci. 2000 Dec;5(12):523-30 PMID: 11120474
  15. A Myb homologue, ATR1, activates tryptophan gene expression in Arabidopsis.
    Proc Natl Acad Sci U S A. 1998 May 12;95(10):5655-60 PMID: 9576939
  16. Expression of a Petunia inflata pectin methyl esterase in Solanum tuberosum L. enhances stem elongation and modifies cation distribution.
    Planta. 2000 Feb;210(3):391-9 PMID: 10750896
  17. Functional dissection of an abscisic acid (ABA)-inducible gene reveals two independent ABA-responsive complexes each containing a G-box and a novel cis-acting element.
    Plant Cell. 1995 Mar;7(3):295-307 PMID: 7734964
  18. cis-acting DNA elements responsive to gibberellin and its antagonist abscisic acid.
    Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7266-70 PMID: 1831269
  19. Two tomato expansin genes show divergent expression and localization in embryos during seed development and germination.
    Plant Physiol. 2001 Nov;127(3):928-36 PMID: 11706175
  20. Genome-wide analysis of core cell cycle genes in Arabidopsis.
    Plant Cell. 2002 Apr;14(4):903-16 PMID: 11971144
  21. The Arabidopsis GA1 locus encodes the cyclase ent-kaurene synthetase A of gibberellin biosynthesis.
    Plant Cell. 1994 Oct;6(10):1509-18 PMID: 7994182
  22. Phase change and the regulation of trichome distribution in Arabidopsis thaliana.
    Development. 1997 Feb;124(3):645-54 PMID: 9043079
  23. A germination-specific endo-beta-mannanase gene is expressed in the micropylar endosperm cap of tomato seeds.
    Plant Physiol. 2000 Aug;123(4):1235-46 PMID: 10938343
  24. Molecular cloning and functional expression of gibberellin 2- oxidases, multifunctional enzymes involved in gibberellin deactivation.
    Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4698-703 PMID: 10200325
  25. Nuclear activation during early germination of the higher plant embryo.
    J Cell Sci. 1985 Apr;75:43-83 PMID: 3900109
  26. The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature.
    Plant Cell Physiol. 2002 Dec;43(12):1421-35 PMID: 12514239
  27. Gibberellin signaling: biosynthesis, catabolism, and response pathways.
    Plant Cell. 2002;14 Suppl:S61-80 PMID: 12045270
  28. The essential Mcm7 protein PROLIFERA is localized to the nucleus of dividing cells during the G(1) phase and is required maternally for early Arabidopsis development.
    Development. 2000 May;127(9):1815-22 PMID: 10751170
  29. Repressing a repressor: gibberellin-induced rapid reduction of the RGA protein in Arabidopsis.
    Plant Cell. 2001 Jul;13(7):1555-66 PMID: 11449051
  30. The alpha-amylase induction in endosperm during rice seed germination is caused by gibberellin synthesized in epithelium.
    Plant Physiol. 2002 Apr;128(4):1264-70 PMID: 11950975
  31. A role for brassinosteroids in germination in Arabidopsis.
    Plant Physiol. 2001 Feb;125(2):763-9 PMID: 11161033
  32. Seed Germination and Dormancy.
    Plant Cell. 1997 Jul;9(7):1055-1066 PMID: 12237375
  33. Induction and analysis of gibberellin sensitive mutants in Arabidopsis thaliana (L.) heynh.
    Theor Appl Genet. 1980 Nov;58(6):257-63 PMID: 24301503
  34. Expression of an ortholog of replication protein A1 (RPA1) is induced by gibberellin in deepwater rice.
    Proc Natl Acad Sci U S A. 1997 Sep 2;94(18):9979-83 PMID: 9275237
  35. Mutations at the SPINDLY locus of Arabidopsis alter gibberellin signal transduction.
    Plant Cell. 1993 Aug;5(8):887-96 PMID: 8400871
  36. Overexpression of a plasma membrane aquaporin in transgenic tobacco improves plant vigor under favorable growth conditions but not under drought or salt stress.
    Plant Cell. 2003 Feb;15(2):439-47 PMID: 12566583
  37. HOW GIBBERELLIN REGULATES PLANT GROWTH AND DEVELOPMENT: A Molecular Genetic Analysis of Gibberellin Signaling.
    Annu Rev Plant Physiol Plant Mol Biol. 2001 Jun;52:67-88 PMID: 11337392
  38. ABSCISIC ACID SIGNAL TRANSDUCTION.
    Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:199-222 PMID: 15012233
  39. Effects of the gibberellin biosynthetic inhibitor uniconazol on mutants of Arabidopsis.
    Plant Physiol. 1991 Oct;97(2):736-8 PMID: 16668460
  40. Expression of an expansin is associated with endosperm weakening during tomato seed germination.
    Plant Physiol. 2000 Nov;124(3):1265-74 PMID: 11080302
  41. Gibberellin-regulated expression of a myb gene in barley aleurone cells: evidence for Myb transactivation of a high-pI alpha-amylase gene promoter.
    Plant Cell. 1995 Nov;7(11):1879-91 PMID: 8535141
  42. FIERY1 encoding an inositol polyphosphate 1-phosphatase is a negative regulator of abscisic acid and stress signaling in Arabidopsis.
    Genes Dev. 2001 Aug 1;15(15):1971-84 PMID: 11485991
  43. Class I beta-1,3-glucanase and chitinase are expressed in the micropylar endosperm of tomato seeds prior to radicle emergence.
    Plant Physiol. 2001 Jul;126(3):1299-313 PMID: 11457981
  44. Regulation of abscisic acid-induced transcription.
    Plant Mol Biol. 1998 Jun;37(3):425-35 PMID: 9617810
  45. Gibberellin biosynthesis: its regulation by endogenous and environmental signals.
    Plant Cell Physiol. 2000 Mar;41(3):251-7 PMID: 10805587
  46. PIF4, a phytochrome-interacting bHLH factor, functions as a negative regulator of phytochrome B signaling in Arabidopsis.
    EMBO J. 2002 May 15;21(10):2441-50 PMID: 12006496
  47. Contrapuntal networks of gene expression during Arabidopsis seed filling.
    Plant Cell. 2002 Jun;14(6):1191-206 PMID: 12084821
  48. Isolation of the GA-response mutant sly1 as a suppressor of ABI1-1 in Arabidopsis thaliana.
    Genetics. 1998 Jun;149(2):509-21 PMID: 9611170
  49. Seed dormancy and germination.
    Curr Opin Plant Biol. 2002 Feb;5(1):33-6 PMID: 11788305
  50. HOOKLESS1, an ethylene response gene, is required for differential cell elongation in the Arabidopsis hypocotyl.
    Cell. 1996 Apr 19;85(2):183-94 PMID: 8612271
  51. Proteomic analysis of arabidopsis seed germination and priming.
    Plant Physiol. 2001 Jun;126(2):835-48 PMID: 11402211
  52. Trp-dependent auxin biosynthesis in Arabidopsis: involvement of cytochrome P450s CYP79B2 and CYP79B3.
    Genes Dev. 2002 Dec 1;16(23):3100-12 PMID: 12464638
  53. Gibberellins regulate seed germination in tomato by endosperm weakening: a study with gibberellin-deficient mutants.
    Planta. 1987 Aug;171(4):525-31 PMID: 24225716
  54. Loosening of plant cell walls by expansins.
    Nature. 2000 Sep 21;407(6802):321-6 PMID: 11014181
  55. Gibberellin regulates Arabidopsis seed germination via RGL2, a GAI/RGA-like gene whose expression is up-regulated following imbibition.
    Genes Dev. 2002 Mar 1;16(5):646-58 PMID: 11877383
  56. Proteomics of Arabidopsis seed germination. A comparative study of wild-type and gibberellin-deficient seeds.
    Plant Physiol. 2002 Jun;129(2):823-37 PMID: 12068122
  57. Action spectra for phytochrome A- and B-specific photoinduction of seed germination in Arabidopsis thaliana.
    Proc Natl Acad Sci U S A. 1996 Jul 23;93(15):8129-33 PMID: 8755615
  58. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.
    Nature. 2000 Dec 14;408(6814):796-815 PMID: 11130711
  59. Gibberellins and Light-Stimulated Seed Germination.
    J Plant Growth Regul. 2001 Dec;20(4):369-376 PMID: 11986762
  60. Endogenous gibberellins in Arabidopsis thaliana and possible steps blocked in the biosynthetic pathways of the semidwarf ga4 and ga5 mutants.
    Proc Natl Acad Sci U S A. 1990 Oct;87(20):7983-7 PMID: 2236013
  61. An increase in pectin methyl esterase activity accompanies dormancy breakage and germination of yellow cedar seeds.
    Plant Physiol. 2000 Sep;124(1):231-42 PMID: 10982438
  62. Xyloglucan endotransglycosylases: diversity of genes, enzymes and potential wall-modifying functions.
    Trends Plant Sci. 1999 Sep;4(9):361-6 PMID: 10462769
  63. SPINDLY, a tetratricopeptide repeat protein involved in gibberellin signal transduction in Arabidopsis.
    Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):9292-6 PMID: 8799194
  64. The new RGA locus encodes a negative regulator of gibberellin response in Arabidopsis thaliana.
    Genetics. 1997 Jul;146(3):1087-99 PMID: 9215910
  65. Arabidopsis RGL1 encodes a negative regulator of gibberellin responses.
    Plant Cell. 2002 Jan;14(1):87-100 PMID: 11826301
  66. A gibberellin-regulated xyloglucan endotransglycosylase gene is expressed in the endosperm cap during tomato seed germination.
    J Exp Bot. 2002 Feb;53(367):215-23 PMID: 11807125
  67. Distinct cell-specific expression patterns of early and late gibberellin biosynthetic genes during Arabidopsis seed germination.
    Plant J. 2001 Nov;28(4):443-53 PMID: 11737781
  68. Two anthranilate synthase genes in Arabidopsis: defense-related regulation of the tryptophan pathway.
    Plant Cell. 1992 Jun;4(6):721-33 PMID: 1392592
  69. Gibberellin requirement for Arabidopsis seed germination is determined both by testa characteristics and embryonic abscisic acid.
    Plant Physiol. 2000 Feb;122(2):415-24 PMID: 10677434
  70. Phytochrome regulation and differential expression of gibberellin 3beta-hydroxylase genes in germinating Arabidopsis seeds.
    Plant Cell. 1998 Dec;10(12):2115-26 PMID: 9836749
  71. Expansins: ever-expanding numbers and functions.
    Curr Opin Plant Biol. 2001 Dec;4(6):527-32 PMID: 11641069
  72. The conserved B3 domain of VIVIPAROUS1 has a cooperative DNA binding activity.
    Plant Cell. 1997 May;9(5):799-807 PMID: 9165754
  73. From genome to function: the Arabidopsis aquaporins.
    Genome Biol. 2002;3(1):RESEARCH0001 PMID: 11806824
  74. Cloning and characterization of an L1 layer-specific gene in Arabidopsis thaliana.
    Plant Cell Physiol. 1999 Jun;40(6):571-80 PMID: 10483121
  75. Pectin methylesterases: cell wall enzymes with important roles in plant physiology.
    Trends Plant Sci. 2001 Sep;6(9):414-9 PMID: 11544130
Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.011650
Published
2003-07-00
Pages
1591-604
Language
English
Region
England
NLM ID
9208688
PMCID
PMC165403
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1105/tpc.011650 PMC Full Text View journal details
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