Abstract
A family of 85/86-kDa (85K/86K) polypeptides closely linked to phosphatidylinositol kinase activity is found in polyoma middle-sized tumor antigen (MTAg)/pp60c-src complexes. MTAg and the 85-kDa phosphoprotein (pp85) could be reassociated in solution, or on blots, after denaturation with SDS. Results from such experiments focus attention on phosphorylation in controlling intracellular sorting and activation of pp85. Tyrosine phosphorylation seems important for recruitment of pp85 from cytosol to membrane. By blotting, pp85 is substantially cytosolic, whereas that recognized by anti-phosphotyrosine antibody is almost exclusively in membranes. Tyrosine phosphorylation also determined association of pp85 with MTAg. Manipulation of MTAg tyrosine phosphorylation, for example, by expressing MTAg using baculovirus vectors in the absence or presence of pp60c-src, dramatically affects reassociation. Finally, tyrosine phosphorylation appears to be involved in release of pp85 from MTAg, since vanadate increased its rate of dissociation.
MeSH Terms
1-Phosphatidylinositol 4-Kinase
Animals
Antigens, Polyomavirus Transforming
Cell Line
Cell Membrane/metabolism
Cell Transformation, Neoplastic
Cells, Cultured
Cytosol/metabolism
Mice
Molecular Weight
Neoplasm Proteins/metabolism
Phosphoproteins/metabolism
Phosphorylation
Phosphotransferases/metabolism
Phosphotyrosine
Polyomavirus/genetics,immunology
Tyrosine/analogs & derivatives,analysis
Chemicals
Antigens, Polyomavirus Transforming
Neoplasm Proteins
Phosphoproteins
Phosphotyrosine
Tyrosine
Phosphotransferases
1-Phosphatidylinositol 4-Kinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cohen B
Department of Biochemistry, Tufts University, Boston, MA 02111.
Yoakim M
Piwnica-Worms H
Roberts T M
Schaffhausen B S
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