Home LiteratureArticle Details
PMID: 1692812 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification, sequencing, and expression of Mycobacterium leprae superoxide dismutase, a major antigen.

Infection and immunity ·Vol. 58 ·No. 6 ·1990-06-00 ·Pages 1937-42

Thangaraj HS, Lamb FI, Davis EO, Jenner PJ, Jeyakumar LH, Colston MJ

Abstract

The gene encoding a major 28-kilodalton antigen of Mycobacterium leprae has now been sequenced and identified as the enzyme superoxide dismutase (SOD) on the basis of the high degree of homology with known SOD sequences. The deduced amino acid sequence shows 67% homology with a human manganese-utilizing SOD and 55% homology with the Escherichia coli manganese-utilizing enzyme. The gene is not expressed from its own promoter in E. coli but is expressed from its own promoter in Mycobacterium smegmatis. The amino acid sequences of epitopes recognized by monoclonal antibodies against the 28-kilodalton antigen have been determined.

MeSH Terms
Amino Acid Sequence Antibodies, Monoclonal/immunology Antigens, Bacterial/genetics Bacterial Proteins/genetics,immunology Base Sequence Cloning, Molecular Epitopes Gene Expression Genes, Bacterial Molecular Sequence Data Mycobacterium leprae/genetics,immunology Restriction Mapping Superoxide Dismutase/genetics,immunology
Chemicals
Antibodies, Monoclonal Antigens, Bacterial Bacterial Proteins Epitopes Superoxide Dismutase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Thangaraj H S
Laboratory for Leprosy and Mycobacterial Research, National Institute for Medical Research, Ridgeway, London, United Kingdom.
Lamb F I
Davis E O
Jenner P J
Jeyakumar L H
Colston M J
References (27)
27 references, click to expand
  1. In vivo repackaging of recombinant cosmid molecules for analyses of Salmonella typhimurium, Streptococcus mutans, and mycobacterial genomic libraries.
    Infect Immun. 1986 Apr;52(1):101-9 PMID: 2937735
  2. Measurements of the effects that coding for a protein has on a DNA sequence and their use for finding genes.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):551-67 PMID: 6364041
  3. The 65-kilodalton antigen of Mycobacterium tuberculosis.
    J Bacteriol. 1987 Mar;169(3):1080-8 PMID: 3029018
  4. Molecular genetics of superoxide dismutases.
    Free Radic Biol Med. 1988;5(5-6):393-402 PMID: 3151488
  5. Stress proteins are immune targets in leprosy and tuberculosis.
    Proc Natl Acad Sci U S A. 1988 Jun;85(12):4267-70 PMID: 3132709
  6. Leprosy: understanding protective immunity.
    Immunol Today. 1989 Jul;10(7):218-21 PMID: 2478144
  7. Monoclonal antibodies to a 28,000 mol. wt protein antigen of Mycobacterium leprae.
    Clin Exp Immunol. 1985 Jun;60(3):546-52 PMID: 2410167
  8. Mycobacterium leprae antigens involved in human immune responses. I. Identification of four antigens by monoclonal antibodies.
    J Immunol. 1985 Dec;135(6):4171-7 PMID: 2415603
  9. Nucleotide and deduced amino acid sequence of Mycobacterium leprae manganese superoxide dismutase.
    Nucleic Acids Res. 1989 Oct 25;17(20):8378 PMID: 2682526
  10. Identification of Mycobacterium leprae antigens in tissues of leprosy patients using monoclonal antibodies.
    Int J Lepr Other Mycobact Dis. 1989 Sep;57(3):652-8 PMID: 2674303
  11. Heterologous expression of the 65-kilodalton antigen of Mycobacterium leprae and murine T-cell responses to the gene product.
    Infect Immun. 1988 May;56(5):1237-41 PMID: 3281904
  12. Iron- and manganese-containing superoxide dismutases can be distinguished by analysis of their primary structures.
    FEBS Lett. 1988 Mar 14;229(2):377-82 PMID: 3345848
  13. The primary structure of human liver manganese superoxide dismutase.
    J Biol Chem. 1984 Oct 25;259(20):12595-601 PMID: 6386798
  14. Evidence for a natural gene transfer from the ponyfish to its bioluminescent bacterial symbiont Photobacter leiognathi. The close relationship between bacteriocuprein and the copper-zinc superoxide dismutase of teleost fishes.
    J Biol Chem. 1981 Jun 25;256(12):6080-9 PMID: 6787049
  15. Superoxide dismutase, peroxidatic activity and catalase in Mycobacterium leprae purified from armadillo liver.
    J Gen Microbiol. 1980 Dec;121(2):457-64 PMID: 7021767
  16. Attempts to establish the armadillo (Dasypus novemcinctus Linn.) as a model for the study of leprosy. I. Report of lepromatoid leprosy in an experimentally infected armadillo.
    Int J Lepr Other Mycobact Dis. 1971 Jul-Sep;39(3):693-702 PMID: 4948218
  17. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  18. Production and characterization of monoclonal antibodies to Mycobacterium tuberculosis, M. bovis (BCG) and M. leprae.
    Clin Exp Immunol. 1984 Dec;58(3):511-21 PMID: 6439449
  19. Aspects of the structure, function, and applications of superoxide dismutase.
    CRC Crit Rev Biochem. 1987;22(2):111-80 PMID: 3315461
  20. Characterization, sequence determination, and immunogenicity of a 64-kilodalton protein of Mycobacterium bovis BCG expressed in escherichia coli K-12.
    Infect Immun. 1987 Jun;55(6):1466-75 PMID: 3553003
  21. Single-stranded DNA 'blue' T7 promoter plasmids: a versatile tandem promoter system for cloning and protein engineering.
    Protein Eng. 1986 Oct-Nov;1(1):67-74 PMID: 3507689
  22. Genes for the major protein antigens of the leprosy parasite Mycobacterium leprae.
    Nature. 1985 Aug 1-7;316(6027):450-2 PMID: 3894979
  23. Molecular analysis of DNA and construction of genomic libraries of Mycobacterium leprae.
    J Bacteriol. 1985 Mar;161(3):1093-102 PMID: 3882664
  24. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  25. Production and partial characterization of monoclonal antibodies to Mycobacterium leprae.
    Infect Immun. 1982 Jul;37(1):172-8 PMID: 6179876
  26. Construction and analysis of in vivo activity of E. coli promoter hybrids and promoter mutants that alter the -35 to -10 spacing.
    Gene. 1982 Dec;20(2):231-43 PMID: 6299890
  27. Lysogeny and transformation in mycobacteria: stable expression of foreign genes.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6987-91 PMID: 2842799
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.6.1937-1942.1990
Published
1990-06-00
Pages
1937-42
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258747
Subset
IM
Databases
GENBANK
X16453
External Links
PubMed source DOI: 10.1128/iai.58.6.1937-1942.1990 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2