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PMID: 16923873 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Pseudomonas syringae HrpJ is a type III secreted protein that is required for plant pathogenesis, injection of effectors, and secretion of the HrpZ1 Harpin.

Journal of bacteriology ·Vol. 188 ·No. 17 ·2006-09-00 ·Pages 6060-9

Fu ZQ, Guo M, Alfano JR

Abstract

The bacterial plant pathogen Pseudomonas syringae requires a type III protein secretion system (TTSS) to cause disease. The P. syringae TTSS is encoded by the hrp-hrc gene cluster. One of the genes within this cluster, hrpJ, encodes a protein with weak similarity to YopN, a type III secreted protein from the animal pathogenic Yersinia species. Here, we show that HrpJ is secreted in culture and translocated into plant cells by the P. syringae pv. tomato DC3000 TTSS. A DC3000 hrpJ mutant, UNL140, was greatly reduced in its ability to cause disease symptoms and multiply in Arabidopsis thaliana. UNL140 exhibited a reduced ability to elicit a hypersensitive response (HR) in nonhost tobacco plants. UNL140 was unable to elicit an AvrRpt2- or AvrB1-dependent HR in A. thaliana but maintained its ability to secrete AvrB1 in culture via the TTSS. Additionally, UNL140 was defective in its ability to translocate the effectors AvrPto1, HopB1, and AvrPtoB. Type III secretion assays showed that UNL140 secreted HrpA1 and AvrPto1 but was unable to secrete HrpZ1, a protein that is normally secreted in culture in relatively large amounts, into culture supernatants. Taken together, our data indicate that HrpJ is a type III secreted protein that is important for pathogenicity and the translocation of effectors into plant cells. Based on the failure of UNL140 to secrete HrpZ1, HrpJ may play a role in controlling type III secretion, and in its absence, specific accessory proteins, like HrpZ1, may not be extracellularly localized, resulting in disabled translocation of effectors into plant cells.

MeSH Terms
Arabidopsis/microbiology Bacterial Outer Membrane Proteins/metabolism Bacterial Proteins/metabolism,physiology Locomotion Plant Diseases/microbiology Pseudomonas syringae/pathogenicity,physiology
Chemicals
Bacterial Outer Membrane Proteins Bacterial Proteins avrPto protein, Pseudomonas syringae hrpJ protein, Pseudomonas syringae HrpZ protein, Pseudomonas syringae
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fu Zheng Qing
Plant Science Initiative, The Beadle Center for Genetic Research, University of Nebraska, 1901 Vine St., Lincoln, NE 68588-0660, USA.
Guo Ming
Alfano James R
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
2006-09-00
Pages
6060-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1595357
Subset
IM
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