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PMID: 16043691 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Comparison of the complete genome sequences of Pseudomonas syringae pv. syringae B728a and pv. tomato DC3000.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 102 ·No. 31 ·2005-08-02 ·Pages 11064-9

Feil H, Feil WS, Chain P, Larimer F, DiBartolo G, Copeland A, Lykidis A, Trong S, Nolan M, Goltsman E, Thiel J, Malfatti S, Loper JE, Lapidus A, Detter JC, Land M, Richardson PM, Kyrpides NC, Ivanova N, Lindow SE

Abstract

The complete genomic sequence of Pseudomonas syringae pv. syringae B728a (Pss B728a) has been determined and is compared with that of P. syringae pv. tomato DC3000 (Pst DC3000). The two pathovars of this economically important species of plant pathogenic bacteria differ in host range and other interactions with plants, with Pss having a more pronounced epiphytic stage of growth and higher abiotic stress tolerance and Pst DC3000 having a more pronounced apoplastic growth habitat. The Pss B728a genome (6.1 Mb) contains a circular chromosome and no plasmid, whereas the Pst DC3000 genome is 6.5 mbp in size, composed of a circular chromosome and two plasmids. Although a high degree of similarity exists between the two sequenced Pseudomonads, 976 protein-encoding genes are unique to Pss B728a when compared with Pst DC3000, including large genomic islands likely to contribute to virulence and host specificity. Over 375 repetitive extragenic palindromic sequences unique to Pss B728a when compared with Pst DC3000 are widely distributed throughout the chromosome except in 14 genomic islands, which generally had lower GC content than the genome as a whole. Content of the genomic islands varies, with one containing a prophage and another the plasmid pKLC102 of Pseudomonas aeruginosa PAO1. Among the 976 genes of Pss B728a with no counterpart in Pst DC3000 are those encoding for syringopeptin, syringomycin, indole acetic acid biosynthesis, arginine degradation, and production of ice nuclei. The genomic comparison suggests that several unique genes for Pss B728a such as ectoine synthase, DNA repair, and antibiotic production may contribute to the epiphytic fitness and stress tolerance of this organism.

MeSH Terms
Bacterial Proteins/genetics,physiology Base Composition DNA Transposable Elements DNA, Bacterial/chemistry,genetics Genome, Bacterial Molecular Sequence Data Plasmids/genetics Prophages/genetics Pseudomonas syringae/classification,genetics,pathogenicity,physiology Species Specificity Virulence/genetics
Chemicals
Bacterial Proteins DNA Transposable Elements DNA, Bacterial
Authors & Affiliations
20 authors, click to expand affiliations / ORCID
Feil Helene
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720, USA.
Feil William S
Chain Patrick
Larimer Frank
DiBartolo Genevieve
Copeland Alex
Lykidis Athanasios
Trong Stephen
Nolan Matt
Goltsman Eugene
Thiel James
Malfatti Stephanie
Loper Joyce E
Lapidus Alla
Detter John C
Land Miriam
Richardson Paul M
Kyrpides Nikos C
Ivanova Natalia
Lindow Steven E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0504930102
Published
2005-08-02
Epub
2005-00-25
Pages
11064-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1182459
Subset
IM
Databases
GENBANK
CP000075
External Links
PubMed source DOI: 10.1073/pnas.0504930102 PMC Full Text View journal details
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