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PMID: 1690576 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Optimizing and characterizing alignment of oriented lipid bilayers containing gramicidin D.

Biophysical journal ·Vol. 57 ·No. 2 ·1990-02-00 ·Pages 351-62

Moll F, Cross TA

Abstract

31P NMR spectroscopy and optical microscopy have been used to characterize samples of gramicidin D in oriented lipid bilayers. Correlations have been made between the defect structures observed under crossed polarizers by optical microscopy and characteristic features of 31P NMR spectra. The sample preparation protocol has been improved using these techniques to achieve minimal dispersion of the bilayer normal and minimal amounts of unoriented sample. The molar ratio of gramicidin to dimyristoyl-phosphatidylcholine, the extent of hydration, and the cosolubilizing solvent system were used as the protocol variables. While hydration level and solvent system had profound effects on the sample orientation the molar ratio did not. However, the 31P chemical shift anisotropy is very sensitive to the molar ratio and can be used as an in situ method for determining the molar ratio.

MeSH Terms
Dimyristoylphosphatidylcholine Gramicidin Lipid Bilayers Magnetic Resonance Spectroscopy/methods Models, Biological Molecular Conformation Phosphorus
Chemicals
Lipid Bilayers Gramicidin Phosphorus Dimyristoylphosphatidylcholine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Moll F
Department of Chemistry, Florida State University, Tallahassee 32306-3006.
Cross T A
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(90)82536-4
Published
1990-02-00
Pages
351-62
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1280675
Subset
IM
Grants
NIAID NIH HHS · AI-23007 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(90)82536-4 PMC Full Text View journal details
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