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PMID: 1682250 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Thromboxane-induced neutrophil adhesion to pulmonary microvascular and aortic endothelium is regulated by CD18.

Inflammation ·Vol. 15 ·No. 3 ·1991-06-00 ·Pages 181-99

Wiles ME, Welbourn R, Goldman G, Hechtman HB, Shepro D

Abstract

Thromboxane (Tx) A2 generation and subsequent selective pulmonary sequestration of neutrophils (PMNs) is characteristic of several forms of the adult respiratory distress syndrome (ARDS). Therefore, we examined PMN-dependent adhesion to cultured pulmonary microvessel and aortic endothelium (EC) in response to U46,619 (Tx mimic). Nonstimulated PMNs were two fold more adherent to pulmonary microvessel EC than to aortic EC (P less than 0.01). PMN pretreatment with Tx mimic (10(-6) M) increased adhesion to both types of EC (P less than 0.01). The Tx mimic-induced adhesion was blocked by receptor antagonists to Tx (SQ29,548) and to leukotrienes (FPL55,712), and by the anti-CD18 mAb TS1/18 (P less than 0.01, all cases). Baseline PMN adhesion also was modulated by Tx, leukotrienes, and CD18, for both EC types. These results indicate pulmonary microvessel EC is intrinsically more adhesive for both nonstimulated and stimulated PMNs than aortic EC and that Tx mediates PMN-dependent adhesion by coupled interaction of Tx and LT receptors via CD18 activation.

MeSH Terms
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid Animals Antigens, CD/immunology Aorta/drug effects,immunology CD18 Antigens Capillaries/immunology Cattle Cell Adhesion/drug effects Cells, Cultured Endothelium, Vascular/drug effects,immunology Leukotriene B4/metabolism Lung/blood supply,immunology Microcirculation/immunology Neutrophils/drug effects,immunology Prostaglandin Endoperoxides, Synthetic/pharmacology Receptors, Immunologic/drug effects Receptors, Leukocyte-Adhesion/immunology Receptors, Leukotriene B4 Thromboxane A2/physiology
Chemicals
Antigens, CD CD18 Antigens Prostaglandin Endoperoxides, Synthetic Receptors, Immunologic Receptors, Leukocyte-Adhesion Receptors, Leukotriene B4 Leukotriene B4 Thromboxane A2 15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wiles M E
Laboratory for Microvascular Research, Biological Science Center, Boston University, Massachusetts 02215.
Welbourn R
Goldman G
Hechtman H B
Shepro D
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Article Info
Journal
Inflammation
Abbr.
Inflammation
ISSN
0360-3997
DOI
10.1007/BF00918645
Published
1991-06-00
Pages
181-99
Language
English
Region
United States
NLM ID
7600105
Subset
IM
Grants
NHLBI NIH HHS · NHLBI 16714 · United States
NHLBI NIH HHS · NHLBI 33104 · United States
NHLBI NIH HHS · NHLBI 43875 · United States
External Links
PubMed source DOI: 10.1007/BF00918645 View journal details
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