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PMID: 1673787 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Poliovirus can enter and infect mammalian cells by way of an intercellular adhesion molecule 1 pathway.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 9 ·1991-05-01 ·Pages 3598-602

Selinka HC, Zibert A, Wimmer E

Abstract

Mouse fibroblast cell lines were transfected with truncated forms of the human poliovirus receptor (PVR) cDNA and tested for the expression of functional receptors for poliovirus. Several receptor constructs, all containing the coding region of the first 143 amino acids of PVR, were able to render mouse cells susceptible to poliovirus infection. A deletion of 65 amino acids in the first extracellular domain of PVR prevented virus attachment and infection. These data suggest that domain 1 is necessary and sufficient for the virus-receptor interaction. A PVR/intercellular adhesion molecule 1 hybrid receptor, expressing the PVR variable domain on a truncated receptor molecule for human rhinovirus 14, was shown to be a functional receptor for poliovirus. This observation indicates that, subsequent to attachment to the PVR-binding domain, poliovirus can use the same pathway as the major receptor group rhinoviruses to enter cells.

MeSH Terms
Animals Base Sequence Cell Adhesion Molecules/physiology Cloning, Molecular DNA Mutational Analysis In Vitro Techniques Intercellular Adhesion Molecule-1 Mice Molecular Sequence Data Oligonucleotides/chemistry Poliovirus/genetics Polymerase Chain Reaction Receptors, Virus/physiology Structure-Activity Relationship Transfection Virus Replication
Chemicals
Cell Adhesion Molecules Oligonucleotides Receptors, Virus Intercellular Adhesion Molecule-1
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Selinka H C
Department of Microbiology, State University of New York, Stony Brook 11794.
Zibert A
Wimmer E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.9.3598
Published
1991-05-01
Pages
3598-602
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51499
Subset
IM
Grants
NIAID NIH HHS · AI-15122 · United States
NCI NIH HHS · CA-28146 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.9.3598 PMC Full Text View journal details
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