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PMID: 1668615 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Macrostructure of the tomato telomeres.

The Plant cell ·Vol. 3 ·No. 1 ·1991-01-00 ·Pages 87-94

Ganal MW, Lapitan NL, Tanksley SD

Abstract

The macrostructure of the tomato telomeres has been investigated by in situ hybridization, genomic sequencing, and pulsed-field gel electrophoresis. In situ hybridizations with a cloned telomeric sequence from Arabidopsis thaliana indicated that the telomeric repeat of tomato cross-hybridizes with that of Arabidopsis and is located at all telomeres. Bal31 digestion kinetics confirmed that the tomato telomeric repeat represents the outermost DNA sequence of each tomato chromosome. Genomic sequencing of enriched tomato telomeric sequences, using primers derived from the Arabidopsis sequence, revealed that the consensus sequence of the tomato telomeric repeat is TT(T/A)AGGG compared with the Arabidopsis consensus sequence of TTTAGGG. Furthermore, as shown by pulsed-field gel electrophoresis, the telomeric repeat of tomato is separated by not more than a few hundred kilobases from a previously described 162-base pair satellite DNA repeat of tomato (TGR I) at 20 of the 24 telomeres. Together, these sequences are found in the heterochromatic terminal knob observed in pachytene chromosomes. Therefore, these two repeats determine the structure of 20 of the 24 tomato chromosome ends over approximately 2% of the total chromosome length.

MeSH Terms
Base Sequence Consensus Sequence DNA/chemistry DNA Restriction Enzymes Molecular Sequence Data Nucleic Acid Hybridization Plants/genetics Repetitive Sequences, Nucleic Acid Telomere/chemistry
Chemicals
DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ganal M W
Department of Plant Breeding and Biometry, Cornell University, Ithaca, New York 14853.
Lapitan N L
Tanksley S D
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.3.1.87
Published
1991-01-00
Pages
87-94
Language
English
Region
England
NLM ID
9208688
PMCID
PMC159981
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.3.1.87 PMC Full Text View journal details
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