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PMID: 16658121 Published · ppublish English Journal Article

Protein synthesis by isolated etioplasts and chloroplasts from pea and wheat and the effects of chloramphenicol and cycloheximide.

Plant physiology ·Vol. 50 ·No. 1 ·1972-07-00 ·Pages 19-23

Reger BJ, Smillie RM, Fuller RC

Abstract

Etioplasts capable of incorporating (14)C-leucine into protein have been isolated from dark-grown pea and wheat plants. The requirements for leucine incorporation for etioplasts were similar to those for chloroplasts. An ATP-generating system, Mg(2+), and GTP were required. The amino-acid-incorporation activity of etioplasts from wheat was comparable to that of chloroplasts on an RNA basis, whereas the activity of pea etioplasts was about 50% of the activity of pea chloroplasts. The incorporation of leucine into protein by etioplasts and chloroplasts from pea and wheat was inhibited by chloramphenicol, and to a slight extent by cycloheximide.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Reger B J
Biology Division, Oak Ridge National Laboratory, The University of Tennessee-Oak Ridge Graduate School of Biomedical Sciences, Oak Ridge, Tennessee 37830.
Smillie R M
Fuller R C
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.50.1.19
Published
1972-07-00
Pages
19-23
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC367308
External Links
PubMed source DOI: 10.1104/pp.50.1.19 PMC Full Text View journal details
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