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PMID: 1665375 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of DNA restriction fragments greater than 5.7 Mb in size from the centromeric region of human chromosomes.

Mammalian genome : official journal of the International Mammalian Genome Society ·Vol. 1 ·No. 4 ·1991-00-00 ·Pages 249-54

Arn PH, Li X, Smith C, Hsu M, Schwartz DC, Jabs EW

Abstract

Pulsed electrophoresis was used to study the organization of the human centromeric region. Genomic DNA was digested with rare-cutting enzymes. DNA fragments from 0.2 to greater than 5.7 Mb were separated by electrophoresis and hybridized with alphoid and simple DNA repeats. Rare-cutting enzymes (Mlu I, Nar I, Not I, Nru I, Sal I, Sfi I, Sst II) demonstrated fewer restriction sites at centromeric regions than elsewhere in the genome. The enzyme Not I had the fewest restriction sites at centromeric regions. As much as 70% of these sequences from the centromeric region are present in Not I DNA fragments greater than 5.7 and estimated to be as large as 10 Mb in size. Other repetitive sequences such as short interspersed repeated segments (SINEs), long interspersed repeated segments (LINEs), ribosomal DNA, and mini-satellite DNA that are not enriched at the centromeric region, are not enriched in Not I fragments of greater than 5.7 Mb in size.

MeSH Terms
Centromere DNA/chemistry,metabolism DNA Restriction Enzymes/metabolism Densitometry Deoxyribonucleases, Type II Site-Specific/metabolism Electrophoresis, Gel, Pulsed-Field Female Humans Male Repetitive Sequences, Nucleic Acid
Chemicals
DNA DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific GCGGCCGC-specific type II deoxyribonucleases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Arn P H
Department of Pediatrics, Johns Hopkins University, Baltimore, Maryland 21205.
Li X
Smith C
Hsu M
Schwartz D C
Jabs E W
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Article Info
Journal
Mammalian genome : official journal of the International Mammalian Genome Society
Abbr.
Mamm Genome
ISSN
0938-8990
DOI
10.1007/BF00352332
Published
1991-00-00
Pages
249-54
Language
English
Region
United States
NLM ID
9100916
Subset
IM
Grants
NICHD NIH HHS · R01 HD22819 · United States
NIGMS NIH HHS · T32 GM7471 · United States
External Links
PubMed source DOI: 10.1007/BF00352332 View journal details
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