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PMID: 16643280 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Clathrin is important for normal actin dynamics and progression of Sla2p-containing patches during endocytosis in yeast.

Traffic (Copenhagen, Denmark) ·Vol. 7 ·No. 5 ·2006-05-00 ·Pages 574-88

Newpher TM, Lemmon SK

Abstract

Clathrin is a major vesicle coat protein involved in receptor-mediated endocytosis. In yeast and higher eukaryotes, clathrin is recruited to the plasma membrane during the early stage of endocytosis along with clathrin-associated adaptors. As coated pits undergo maturation, a burst of actin polymerization accompanies and helps drive vesicle internalization. Here, we investigate the dynamics of clathrin relative to the early endocytic patch protein Sla2p. We find that clathrin is recruited to the cortex prior to Sla2p. In the absence of clathrin, normal numbers of Sla2p patches form, but many do not internalize or are dramatically delayed in completion of endocytosis. Patches that do internalize receive Sla1p late, which is followed by Abp1, which appears near the end of Sla2p lifetime. In addition, clathrin mutants develop actin comet tails, suggesting an important function in actin patch organization/dynamics. Similar to its mammalian counterparts, the light chain (LC) subunit of yeast clathrin interacts directly with the coiled-coil domain of Sla2p. A mutant of Sla2p that no longer interacts with LC (sla2Delta376-573) results in delayed progression of endocytic patches and aberrant actin dynamics. These data demonstrate an important role for clathrin in organization and progression of early endocytic patches to the late stages of endocytosis.

MeSH Terms
Actins/metabolism Carrier Proteins/metabolism Clathrin/physiology Cytoskeletal Proteins Endocytosis/physiology Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins/metabolism
Chemicals
Actins Carrier Proteins Clathrin Cytoskeletal Proteins SLA2 protein, S cerevisiae Saccharomyces cerevisiae Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Newpher Thomas M
Department of Molecular Biology and Microbiology, Case Western Reserve University, Cleveland, OH 44106, USA.
Lemmon Sandra K
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Article Info
Journal
Traffic (Copenhagen, Denmark)
Abbr.
Traffic
ISSN
1398-9219
DOI
10.1111/j.1600-0854.2006.00410.x
Published
2006-05-00
Pages
574-88
Language
English
Region
England
NLM ID
100939340
PMCID
PMC2975023
Subset
IM
Grants
NIGMS NIH HHS · R01 GM055796 · United States
NIGMS NIH HHS · R01 GM055796-09 · United States
PHS HHS · R01 G55796 · United States
PHS HHS · T32 G08056-20 · United States
External Links
PubMed source DOI: 10.1111/j.1600-0854.2006.00410.x PMC Full Text View journal details
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