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PMID: 11847108 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dynamin and clathrin are required for the biogenesis of a distinct class of secretory vesicles in yeast.

The EMBO journal ·Vol. 21 ·No. 4 ·2002-02-15 ·Pages 602-14

Gurunathan S, David D, Gerst JE

Abstract

Yeast produce two classes of secretory vesicles (SVs) that differ in both density and cargo protein content. In late-acting secretory mutants (e.g. snc1(ala43) and sec6-4), both low- (LDSV) and high-density (HDSV) classes of vesicles accumulate at restrictive temperatures. Here, we have found that disruptions in the genes encoding a dynamin-related protein (VPS1) or clathrin heavy chain (CHC1) abolish HDSV production, yielding LDSVs that contain all secreted cargos. Interestingly, disruption of the PEP12 gene, which encodes the t-SNARE that mediates all Golgi to pre-vacuolar compartment (PVC) transport, also abolishes HDSV production. In contrast, deletions in genes that selectively confer vacuolar hydrolase sorting to the PVC or protein transport to the vacuole (i.e. VPS34 and VAM3, respectively) have no effect. Thus, one branch of the secretory pathway in yeast involves an intermediate sorting compartment and has a specific requirement for clathrin and a dynamin-related protein in SV biogenesis.

MeSH Terms
Carrier Proteins/genetics Clathrin/physiology Dynamins Endosomes/metabolism GTP Phosphohydrolases/physiology GTP-Binding Proteins Genes, Fungal Golgi Apparatus/metabolism Microscopy, Electron Mutation Organelles/ultrastructure Saccharomyces cerevisiae/genetics,metabolism,ultrastructure Temperature Vesicular Transport Proteins
Chemicals
Carrier Proteins Clathrin Vesicular Transport Proteins GTP Phosphohydrolases GTP-Binding Proteins VPS1 protein, S cerevisiae Dynamins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gurunathan Sangiliyandi
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.
David Doris
Gerst Jeffrey E
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1093/emboj/21.4.602
Published
2002-02-15
Pages
602-14
Language
English
Region
England
NLM ID
8208664
PMCID
PMC125853
Subset
IM
External Links
PubMed source DOI: 10.1093/emboj/21.4.602 PMC Full Text View journal details
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