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PMID: 16617483 Published · ppublish English Journal Article

Potential role of proteolysis in the control of UvrABC incision.

Nucleic acids research ·Vol. 16 ·No. 20 ·1988-10-25 ·Pages 9641-50

Caron PR, Grossman L

Abstract

UvrB is specifically proteolyzed in Escherichia coli cell extracts to UvrB*. UvrB* is capable of interacting with UvrA in an aparently similar manner to the UvrB, however UvrB* is defective in the DNA strand displacement activity normally displayed by UvrAB. Whereas the binding of UvrC to a UvrAB-DNA complex leads to DNA incision and persistence of a stable post-incision protein-DNA complex, the binding of UvrC to UvrAB* leads to dissociation of the protein complex and no DNA incision is seen. The factor which stimulates this proteolysis has been partially purified and its substrate specificity has been examined. The protease factor is induced by "stress" and is under control of the htpR gene. The potential role of this proteolysis in the regulation of levels of active repair enzymes in the cell is discussed.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Caron P R
Department of Biochemistry, The Johns Hopkins University, School of Hygiene and Public Health, 615 North Wolfe Street, Baltimore, MD 21205, USA.
Grossman L
References (42)
42 references, click to expand
  1. RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis.
    Proc Natl Acad Sci U S A. 1988 Mar;85(6):1806-10 PMID: 3126496
  2. Proteolysis and physiological regulation.
    Mol Aspects Med. 1987;9(3):173-287 PMID: 3295447
  3. Inhibitory effect of membrane-binding drugs on excision repair of DNA damage in UV-irradiated Escherichia coli.
    Mutat Res. 1983 Apr;112(2):97-107 PMID: 6339887
  4. The modulating influence of the fluidity of cell membrane on excision repair of DNA in UV-irradiated Escherichia coli.
    Biochem Biophys Res Commun. 1983 Jan 27;110(2):609-15 PMID: 6340668
  5. Functional domains and methyl acceptor sites of the Escherichia coli ada protein.
    J Biol Chem. 1988 Mar 25;263(9):4430-3 PMID: 3162236
  6. Sequences of the E. coli uvrB gene and protein.
    Nucleic Acids Res. 1986 Mar 25;14(6):2637-50 PMID: 3515321
  7. Relation of the Escherichia coli dnaX gene to its two products--the tau and gamma subunits of DNA polymerase III holoenzyme.
    Nucleic Acids Res. 1987 Oct 12;15(19):7663-75 PMID: 3313272
  8. Ability of various alkylating agents to induce adaptive and SOS responses: a study with lacZ fusion.
    Mutat Res. 1985 Sep;146(2):149-54 PMID: 2993877
  9. Incision of damaged versus nondamaged DNA by the Escherichia coli UvrABC proteins.
    Nucleic Acids Res. 1988 Aug 25;16(16):7855-65 PMID: 2843804
  10. A class of Escherichia coli proteins controlled by the hflA locus.
    J Mol Biol. 1987 Aug 5;196(3):737-40 PMID: 2824788
  11. Nucleotide sequence of the Escherichia coli replication gene dnaZX.
    Nucleic Acids Res. 1986 Aug 26;14(16):6541-9 PMID: 3018672
  12. Regulatory mechanisms for induction of synthesis of repair enzymes in response to alkylating agents: ada protein acts as a transcriptional regulator.
    Proc Natl Acad Sci U S A. 1986 Sep;83(17):6297-301 PMID: 3529081
  13. A new membrane-associated DNA replication protein, the gene 69 product of bacteriophage T4, shares a patch of homology with the Escherichia coli dnaA protein.
    J Mol Biol. 1986 May 5;189(1):243-8 PMID: 3023622
  14. The effect of Escherichia coli Uvr protein binding on the topology of supercoiled DNA.
    Nucleic Acids Res. 1986 Nov 11;14(21):8557-71 PMID: 3024109
  15. Helicase properties of the Escherichia coli UvrAB protein complex.
    Proc Natl Acad Sci U S A. 1987 Jun;84(11):3638-42 PMID: 3035542
  16. The yeast DNA repair gene RAD6 encodes a ubiquitin-conjugating enzyme.
    Nature. 1987 Sep 10-16;329(6135):131-4 PMID: 3306404
  17. Repair of N-methyl-N'-nitro-N-nitrosoguanidine-induced DNA damage by ABC excinuclease.
    J Bacteriol. 1987 Feb;169(2):540-5 PMID: 3542961
  18. Induction and autoregulation of ada, a positively acting element regulating the response of Escherichia coli K-12 to methylating agents.
    J Bacteriol. 1985 Mar;161(3):888-95 PMID: 2982792
  19. The intracellular signal for induction of resistance to alkylating agents in E. coli.
    Cell. 1986 Apr 25;45(2):315-24 PMID: 3009022
  20. Evidence for RecA protein association with the cell membrane and for changes in the levels of major outer membrane proteins in SOS-induced Escherichia coli cells.
    J Bacteriol. 1985 Sep;163(3):870-6 PMID: 3897198
  21. Nucleotide sequence of Bacillus subtilis dnaB: a gene essential for DNA replication initiation and membrane attachment.
    Proc Natl Acad Sci U S A. 1987 Feb;84(3):653-7 PMID: 3027697
  22. Cell-division control in Escherichia coli: specific induction of the SOS function SfiA protein is sufficient to block septation.
    Proc Natl Acad Sci U S A. 1984 Jul;81(14):4490-4 PMID: 6087326
  23. Protein complexes formed during the incision reaction catalyzed by the Escherichia coli UvrABC endonuclease.
    Nucleic Acids Res. 1986 Mar 25;14(6):2567-82 PMID: 3960727
  24. Induction of SOS and adaptive responses by alkylating agents in Escherichia coli mutants deficient in 3-methyladenine-DNA glycosylase activities.
    Mutat Res. 1987 Jan;183(1):11-20 PMID: 3099190
  25. Active site and complete sequence of the suicidal methyltransferase that counters alkylation mutagenesis.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2688-92 PMID: 3887409
  26. Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli.
    Microbiol Rev. 1984 Mar;48(1):60-93 PMID: 6371470
  27. Ubiquitin has intrinsic proteolytic activity: implications for cellular regulation.
    Proc Natl Acad Sci U S A. 1987 Jun;84(11):3685-9 PMID: 3035547
  28. UmuD mutagenesis protein of Escherichia coli: overproduction, purification, and cleavage by RecA.
    Proc Natl Acad Sci U S A. 1988 Mar;85(6):1811-5 PMID: 3279417
  29. The SOS regulatory system: control of its state by the level of RecA protease.
    J Mol Biol. 1983 Jul 15;167(4):791-808 PMID: 6410076
  30. Proteolytic processing of the Ada protein that repairs DNA O6-methylguanine residues in E. coli.
    Mutat Res. 1987 Mar;183(2):123-7 PMID: 3547105
  31. Enzymatic properties of purified Escherichia coli uvrABC proteins.
    Proc Natl Acad Sci U S A. 1983 Oct;80(20):6157-61 PMID: 6312446
  32. Binding of the origin of replication of Escherichia coli to the outer membrane.
    Cell. 1982 Oct;30(3):915-23 PMID: 6754093
  33. Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli.
    J Bacteriol. 1987 Jan;169(1):26-32 PMID: 3539918
  34. The adjacent dnaZ and dnaX genes of Escherichia coli are contained within one continuous open reading frame.
    Nucleic Acids Res. 1986 Oct 24;14(20):8091-101 PMID: 3534795
  35. Purification and properties of the uvrA protein from Escherichia coli.
    Proc Natl Acad Sci U S A. 1982 Feb;79(4):988-92 PMID: 6280177
  36. RecA-mediated cleavage activates UmuD for mutagenesis: mechanistic relationship between transcriptional derepression and posttranslational activation.
    Proc Natl Acad Sci U S A. 1988 Mar;85(6):1816-20 PMID: 3279418
  37. groEL and dnaK genes of Escherichia coli are induced by UV irradiation and nalidixic acid in an htpR+-dependent fashion.
    Proc Natl Acad Sci U S A. 1984 Mar;81(5):1499-503 PMID: 6324197
  38. A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged region.
    Cell. 1983 May;33(1):249-60 PMID: 6380755
  39. Amplification and purification of UvrA, UvrB, and UvrC proteins of Escherichia coli.
    J Biol Chem. 1985 Aug 15;260(17):9875-83 PMID: 2991268
  40. The purification of the Escherichia coli UvrABC incision system.
    Nucleic Acids Res. 1986 Nov 11;14(21):8535-56 PMID: 3024108
  41. Sigma 32 synthesis can regulate the synthesis of heat shock proteins in Escherichia coli.
    Genes Dev. 1987 Apr;1(2):179-84 PMID: 3315848
  42. Protein degradation in Escherichia coli: the lon gene controls the stability of sulA protein.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):358-62 PMID: 6300834
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.20.9641
Published
1988-10-25
Pages
9641-50
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338769
Corrections
RepublishedIn
PMID: 3060851
External Links
PubMed source DOI: 10.1093/nar/16.20.9641 PMC Full Text View journal details
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