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PMID: 16593578 Published · ppublish English Journal Article

Cloning and expression of cDNA for salmon growth hormone in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 13 ·1985-07-00 ·Pages 4306-10

Sekine S, Mizukami T, Nishi T, Kuwana Y, Saito A, Sato M, Itoh S, Kawauchi H

Abstract

cDNA clones encoding chum salmon (Oncorhynchus keta) growth hormone (sGH) have been isolated from a cDNA library prepared from chum salmon pituitary gland poly(A)(+) RNA. Synthetic oligodeoxynucleotide mixtures based on amino acid residues 23-28 of sGH were used as hybridization probes to select recombinant plasmids carrying the sGH coding sequence. The complete nucleotide sequence of sGH cDNA has been determined. The cDNA sequence codes for a polypeptide of 210 amino acids, including a putative signal sequence of 22 amino acids. The 5' and 3' untranslated regions of the message were 64 and 426 bases long, respectively. Mature sGH was efficiently expressed in Escherichia coli carrying a plasmid in which the sGH cDNA was under control of the E. coli trp promoter; sGH comprised about 15% of the total cellular protein in such bacteria. The partially purified sGH from E. coli stimulated the growth of rainbow trout and the activity was indistinguishable from that of natural sGH.

Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Sekine S
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd. 3-6-6, Asahimachi, Machidashi, Tokyo 194, Japan.
Mizukami T
Nishi T
Kuwana Y
Saito A
Sato M
Itoh S
Kawauchi H
References (23)
23 references, click to expand
  1. Efficient bacterial expression of bovine and porcine growth hormones.
    DNA. 1983;2(1):37-45 PMID: 6303731
  2. Development and validation of a carp growth hormone radioimmunoassay.
    Gen Comp Endocrinol. 1983 Jun;50(3):335-47 PMID: 6193032
  3. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments.
    Gene. 1982 Oct;19(3):269-76 PMID: 6295880
  4. Structure of cloned DNA complementary to rat prolactin messenger RNA.
    J Biol Chem. 1980 Jul 10;255(13):6502-10 PMID: 6993473
  5. Molecular cloning of DNA complementary to bovine growth hormone mRNA.
    J Biol Chem. 1980 Aug 25;255(16):7521-4 PMID: 6893197
  6. Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.
    Nucleic Acids Res. 1982 Mar 11;10(5):1755-69 PMID: 7071021
  7. Characteristics of growth hormone isolated from sturgeon (Acipenser güldenstädti) pituitaries.
    Endocrinology. 1981 Feb;108(2):377-81 PMID: 7449727
  8. Direct expression in Escherichia coli of a DNA sequence coding for human growth hormone.
    Nature. 1979 Oct 18;281(5732):544-8 PMID: 386136
  9. Human growth hormone: complementary DNA cloning and expression in bacteria.
    Science. 1979 Aug 10;205(4406):602-7 PMID: 377496
  10. Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone.
    Nature. 1977 Dec 8;270(5637):486-94 PMID: 339105
  11. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  12. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  13. Construction and analysis of recombinant DNA for human chorionic somatomammotropin.
    Nature. 1977 Dec 8;270(5637):494-9 PMID: 593368
  14. Purification and properties of teleost growth hormone.
    Gen Comp Endocrinol. 1976 Sep;30(1):91-100 PMID: 825412
  15. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  16. The chemistry of growth hormone and the lactogenic hormones.
    Recent Prog Horm Res. 1973;29:387-416 PMID: 4584368
  17. Sequences of pituitary and placental lactogenic and growth hormones: evolution from a primordial peptide by gene reduplication.
    Proc Natl Acad Sci U S A. 1971 Apr;68(4):866-70 PMID: 5279528
  18. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  19. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  20. The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.
    Nucleic Acids Res. 1981 Feb 25;9(4):879-94 PMID: 7232206
  21. A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.
    Mol Cell Biol. 1983 Feb;3(2):280-9 PMID: 6300662
  22. Isolation and properties of chum salmon prolactin.
    Gen Comp Endocrinol. 1983 Mar;49(3):446-58 PMID: 6188649
  23. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.13.4306
Published
1985-07-00
Pages
4306-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390402
External Links
PubMed source DOI: 10.1073/pnas.82.13.4306 PMC Full Text View journal details
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