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PMID: 1655565 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Histone regulatory (hir) mutations suppress delta insertion alleles in Saccharomyces cerevisiae.

Genetics ·Vol. 128 ·No. 4 ·1991-08-00 ·Pages 729-38

Sherwood PW, Osley MA

Abstract

Changes in histone gene dosage as well as mutations within some histone genes suppress delta insertion mutations in the HIS4 and LYS2 loci of Saccharomyces cerevisiae by altering the site of transcription initiation. We have found that three histone regulatory (hir) mutations, identified by their effects on the regulation of histone gene expression, suppress the same insertion mutations. In addition, we have examined whether any previously identified spt (suppressor of Ty) mutations might suppress the delta insertion alleles because of effects on histone gene regulation. Our results demonstrate that mutations in the histone genes SPT11/HTA1 and SPT12/HTB1 and in three other SPT genes, SPT1, SPT10 and SPT21, confer Hir- phenotypes. The spt1 mutation was found to be an allele of HIR2 while the spt10 and spt21 mutations are not in any of the known HIR genes.

Related Genes
HIR SPT1 SPT10 SPT11/HTA1 SPT12/HTB1 SPT21
MeSH Terms
Alleles Blotting, Northern DNA Transposable Elements Gene Expression Regulation, Fungal Genes, Fungal/genetics Genes, Recessive Genes, Regulator Histones/genetics Multigene Family Saccharomyces cerevisiae/genetics Suppression, Genetic Transcription, Genetic/genetics Transcriptional Activation
Chemicals
DNA Transposable Elements Histones
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sherwood P W
Program in Molecular Biology, Sloan Kettering Cancer Center, New York, New York.
Osley M A
References (34)
34 references, click to expand
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/128.4.729
Published
1991-08-00
Pages
729-38
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1204547
Subset
IM
Grants
NIGMS NIH HHS · R01 GM040118 · United States
NIGMS NIH HHS · GM-40118 · United States
External Links
PubMed source DOI: 10.1093/genetics/128.4.729 PMC Full Text View journal details
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