Home LiteratureArticle Details
PMID: 16543219 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

The Saccharomyces cerevisiae histone H2A variant Htz1 is acetylated by NuA4.

Genes & development ·Vol. 20 ·No. 6 ·2006-03-15 ·Pages 660-5

Keogh MC, Mennella TA, Sawa C, Berthelet S, Krogan NJ, Wolek A, Podolny V, Carpenter LR, Greenblatt JF, Baetz K, Buratowski S

Abstract

The histone H2A variant H2A.Z (Saccharomyces cerevisiae Htz1) plays roles in transcription, DNA repair, chromosome stability, and limiting telomeric silencing. The Swr1-Complex (SWR-C) inserts Htz1 into chromatin and shares several subunits with the NuA4 histone acetyltransferase. Furthermore, mutants of these two complexes share several phenotypes, suggesting they may work together. Here we show that NuA4 acetylates Htz1 Lys 14 (K14) after the histone is assembled into chromatin by the SWR-C. K14 mutants exhibit specific defects in chromosome transmission without affecting transcription, telomeric silencing, or DNA repair. Function-specific modifications may help explain how the same component of chromatin can function in diverse pathways.

MeSH Terms
Acetylation Acetyltransferases/metabolism Amino Acid Sequence Chromosomes, Fungal Histone Acetyltransferases Histones/chemistry,metabolism Molecular Sequence Data Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins/chemistry,metabolism Sequence Homology, Amino Acid
Chemicals
Histones Htz1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Acetyltransferases Histone Acetyltransferases NuA4 protein, S cerevisiae
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Keogh Michael-Christopher
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Mennella Thomas A
Sawa Chika
Berthelet Sharon
Krogan Nevan J
Wolek Adam
Podolny Vladimir
Carpenter Laura Rocco
Greenblatt Jack F
Baetz Kristin
Buratowski Stephen
References (40)
40 references, click to expand
  1. Analysis of a histone H2A variant from fission yeast: evidence for a role in chromosome stability.
    Mol Gen Genet. 1994 Dec 1;245(5):628-35 PMID: 7808414
  2. ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex.
    Science. 2004 Jan 16;303(5656):343-8 PMID: 14645854
  3. A novel histone H4 mutant defective in nuclear division and mitotic chromosome transmission.
    Mol Cell Biol. 1996 Mar;16(3):1017-26 PMID: 8622646
  4. ESA1 is a histone acetyltransferase that is essential for growth in yeast.
    Proc Natl Acad Sci U S A. 1998 Mar 31;95(7):3561-5 PMID: 9520405
  5. Cse4p is a component of the core centromere of Saccharomyces cerevisiae.
    Cell. 1998 Sep 4;94(5):607-13 PMID: 9741625
  6. Histone acetyltransferases: preparation of substrates and assay procedures.
    Methods Enzymol. 1999;304:675-96 PMID: 10372390
  7. NuA4, an essential transcription adaptor/histone H4 acetyltransferase complex containing Esa1p and the ATM-related cofactor Tra1p.
    EMBO J. 1999 Sep 15;18(18):5108-19 PMID: 10487762
  8. Systematic yeast synthetic lethal and synthetic dosage lethal screens identify genes required for chromosome segregation.
    Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):13956-61 PMID: 16172405
  9. The replacement histone H2A.Z in a hyperacetylated form is a feature of active genes in the chicken.
    Nucleic Acids Res. 2005;33(17):5633-9 PMID: 16204459
  10. Histone variant H2A.Z marks the 5' ends of both active and inactive genes in euchromatin.
    Cell. 2005 Oct 21;123(2):233-48 PMID: 16239142
  11. Diverse functions of spindle assembly checkpoint genes in Saccharomyces cerevisiae.
    Genetics. 2006 Jan;172(1):53-65 PMID: 16157669
  12. The language of covalent histone modifications.
    Nature. 2000 Jan 6;403(6765):41-5 PMID: 10638745
  13. Histone-histone interactions and centromere function.
    Mol Cell Biol. 2000 Aug;20(15):5700-11 PMID: 10891506
  14. Histone H2A.Z regulats transcription and is partially redundant with nucleosome remodeling complexes.
    Cell. 2000 Oct 27;103(3):411-22 PMID: 11081628
  15. Histone H2A.Z acetylation modulates an essential charge patch.
    Mol Cell. 2001 Jun;7(6):1329-35 PMID: 11430834
  16. Kinetochore sub-structure comes to MIND.
    Nat Cell Biol. 2004 Feb;6(2):94-5 PMID: 14755271
  17. A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin.
    PLoS Biol. 2004 May;2(5):E131 PMID: 15045029
  18. Structures of protein domains that create or recognize histone modifications.
    EMBO Rep. 2004 May;5(5):464-9 PMID: 15184976
  19. RNA interference demonstrates a novel role for H2A.Z in chromosome segregation.
    Nat Struct Mol Biol. 2004 Jul;11(7):650-5 PMID: 15195148
  20. Regulation of chromosome stability by the histone H2A variant Htz1, the Swr1 chromatin remodeling complex, and the histone acetyltransferase NuA4.
    Proc Natl Acad Sci U S A. 2004 Sep 14;101(37):13513-8 PMID: 15353583
  21. Quantitative determination of histone modification. H2A acetylation and phosphorylation.
    J Biol Chem. 1981 May 10;256(9):4669-75 PMID: 7217105
  22. Butyrate-induced histone hyperacetylation in human and mouse cells: estimation of putative sites of histone acetylation in vivo.
    J Cell Biochem. 1982;20(3):225-35 PMID: 7169496
  23. Visual assay for chromosome ploidy.
    Methods Enzymol. 1987;155:351-72 PMID: 3431466
  24. Nucleosome depletion alters the chromatin structure of Saccharomyces cerevisiae centromeres.
    Mol Cell Biol. 1990 Nov;10(11):5721-7 PMID: 2233714
  25. Silent domains are assembled continuously from the telomere and are defined by promoter distance and strength, and by SIR3 dosage.
    Genes Dev. 1993 Jul;7(7A):1133-45 PMID: 8319906
  26. Translating the histone code.
    Science. 2001 Aug 10;293(5532):1074-80 PMID: 11498575
  27. Yng2p-dependent NuA4 histone H4 acetylation activity is required for mitotic and meiotic progression.
    J Biol Chem. 2001 Nov 23;276(47):43653-62 PMID: 11544250
  28. Histone acetylation: a switch between repressive and permissive chromatin. Second in review series on chromatin dynamics.
    EMBO Rep. 2002 Mar;3(3):224-9 PMID: 11882541
  29. Chromosome cohesion and separation: from men and molecules.
    Curr Biol. 2003 Feb 4;13(3):R104-14 PMID: 12573239
  30. Chromatin proteins are determinants of centromere function.
    Curr Top Microbiol Immunol. 2003;274:23-52 PMID: 12596903
  31. Centromeres and kinetochores: from epigenetics to mitotic checkpoint signaling.
    Cell. 2003 Feb 21;112(4):407-21 PMID: 12600307
  32. Different sensitivities of bromodomain factors 1 and 2 to histone H4 acetylation.
    Mol Cell. 2003 Feb;11(2):353-63 PMID: 12620224
  33. Conserved histone variant H2A.Z protects euchromatin from the ectopic spread of silent heterochromatin.
    Cell. 2003 Mar 7;112(5):725-36 PMID: 12628191
  34. Finding functional features in Saccharomyces genomes by phylogenetic footprinting.
    Science. 2003 Jul 4;301(5629):71-6 PMID: 12775844
  35. Yaf9, a novel NuA4 histone acetyltransferase subunit, is required for the cellular response to spindle stress in yeast.
    Mol Cell Biol. 2003 Sep;23(17):6086-102 PMID: 12917332
  36. Bur1 kinase is required for efficient transcription elongation by RNA polymerase II.
    Mol Cell Biol. 2003 Oct;23(19):7005-18 PMID: 12972617
  37. Structure, function, and regulation of budding yeast kinetochores.
    Annu Rev Cell Dev Biol. 2003;19:519-39 PMID: 14570580
  38. Phylogenomics of the nucleosome.
    Nat Struct Biol. 2003 Nov;10(11):882-91 PMID: 14583738
  39. A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1.
    Mol Cell. 2003 Dec;12(6):1565-76 PMID: 14690608
  40. Histone H4 and the maintenance of genome integrity.
    Genes Dev. 1995 Jul 15;9(14):1716-27 PMID: 7622036
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1388106
Published
2006-03-15
Pages
660-5
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1413285
Subset
IM
Grants
NIGMS NIH HHS · R01 GM046498 · United States
NIGMS NIH HHS · GM46498 · United States
External Links
PubMed source DOI: 10.1101/gad.1388106 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2