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PMID: 16157669 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Diverse functions of spindle assembly checkpoint genes in Saccharomyces cerevisiae.

Genetics ·Vol. 172 ·No. 1 ·2006-01-00 ·Pages 53-65

Daniel JA, Keyes BE, Ng YP, Freeman CO, Burke DJ

Abstract

The spindle assembly checkpoint regulates the metaphase-to-anaphase transition from yeast to humans. We examined the genetic interactions with four spindle assembly checkpoint genes to identify nonessential genes involved in chromosome segregation, to identify the individual roles of the spindle assembly checkpoint genes within the checkpoint, and to reveal potential complexity that may exist. We used synthetic genetic array (SGA) analysis using spindle assembly checkpoint mutants mad1, mad2, mad3, and bub3. We found 228 synthetic interactions with the four spindle assembly checkpoint mutants with substantial overlap in the spectrum of interactions between mad1, mad2, and bub3. In contrast, there were many synthetic interactions that were common to mad1, mad2, and bub3 that were not shared by mad3. We found shared interactions between pairs of spindle assembly checkpoint mutants, suggesting additional complexity within the checkpoint and unique interactions for all of the spindle assembly checkpoint genes. We show that most genes in the interaction network, including ones with unique interactions, affect chromosome transmission or microtubule function, suggesting that the complexity of interactions reflects diverse roles for the checkpoint genes within the checkpoint. Our analysis expands our understanding of the spindle assembly checkpoint and identifies new candidate genes with possible roles in chromosome transmission and mitotic spindle function.

MeSH Terms
Cell Cycle Proteins/genetics,metabolism Chromosome Segregation Chromosomes, Fungal/physiology Fungal Proteins/genetics,metabolism Gene Expression Regulation, Fungal Mad2 Proteins Microtubules/physiology Mitosis Neural Networks, Computer Nuclear Proteins/genetics,metabolism Oligonucleotide Array Sequence Analysis Phosphorylation Protein Kinases/genetics,metabolism Protein Serine-Threonine Kinases Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Spindle Apparatus/physiology
Chemicals
Cell Cycle Proteins Fungal Proteins MAD1 protein, S cerevisiae MAD2 protein, S cerevisiae MAD3 protein, S cerevisiae Mad2 Proteins Nuclear Proteins Saccharomyces cerevisiae Proteins Protein Kinases Bub1 spindle checkpoint protein Protein Serine-Threonine Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Daniel Jewel A
Department of Biochemistry and Molecular Genetics, University of Virginia Medical Center, Charlottesville, Virginia 22908, USA.
Keyes Brice E
Ng Yvonne P Y
Freeman C Onyi
Burke Daniel J
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1534/genetics.105.046441
Published
2006-01-00
Epub
2005-00-12
Pages
53-65
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1456180
Subset
IM
Grants
NIGMS NIH HHS · F31 GM065070 · United States
NIGMS NIH HHS · R01 GM40334 · United States
NIGMS NIH HHS · F31 GM 65070 · United States
NIGMS NIH HHS · R01 GM040334 · United States
NCI NIH HHS · R01 CA 99036 · United States
NCI NIH HHS · R01 CA099036 · United States
External Links
PubMed source DOI: 10.1534/genetics.105.046441 PMC Full Text View journal details
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