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PMID: 1649390 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Constant expression and activity of protein phosphatase 2A in synchronized cells.

Molecular and cellular biology ·Vol. 11 ·No. 8 ·1991-08-00 ·Pages 4282-5

Ruediger R, Van Wart Hood JE, Mumby M, Walter G

Abstract

The levels of the A, B, and C subunits of protein phosphatase 2A in extracts from synchronized embryonic bovine tracheal cells were determined by immunoblotting with subunit-specific antibodies. A constant amount of each subunit was found in resting cells as well as in growing cells from all stages of the cell cycle. The phosphatase activity of protein phosphatase 2A was also constant. A quantitative comparison showed that the A and C subunits were present in similar amounts, whereas the B subunit was present at a significantly lower level. Together, the A, B, and C subunits represented approximately 0.2% of the total cellular protein.

MeSH Terms
Animals Cattle Cell Cycle Cells, Cultured Embryo, Mammalian Kinetics Macromolecular Substances Muscle, Smooth/cytology,enzymology Phosphoprotein Phosphatases/biosynthesis,isolation & purification,metabolism Protein Phosphatase 2 Trachea/cytology,enzymology
Chemicals
Macromolecular Substances Phosphoprotein Phosphatases Protein Phosphatase 2
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ruediger R
Department of Pathology, University of California, San Diego, La Jolla 92093-0612.
Van Wart Hood J E
Mumby M
Walter G
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.8.4282-4285.1991
Published
1991-08-00
Pages
4282-5
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC361261
Subset
IM
Grants
NCI NIH HHS · CA-21327 · United States
NHLBI NIH HHS · HL-17669 · United States
NHLBI NIH HHS · HL-31107 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.8.4282-4285.1991 PMC Full Text View journal details
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