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PMID: 16397291 Published · epublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Validation Study

Fast chromatin immunoprecipitation assay.

Nucleic acids research ·Vol. 34 ·No. 1 ·2006-01-05 ·Pages e2

Nelson JD, Denisenko O, Sova P, Bomsztyk K

Abstract

Chromatin immunoprecipitation (ChIP) is a widely used method to explore in vivo interactions between proteins and DNA. The ChIP assay takes several days to complete, involves several tube transfers and uses either phenol-chlorophorm or spin columns to purify DNA. The traditional ChIP method becomes a challenge when handling multiple samples. We have developed an efficient and rapid Chelex resin-based ChIP procedure that dramatically reduces time of the assay and uses only a single tube to isolate PCR-ready DNA. This method greatly facilitates the probing of chromatin changes over many time points with several antibodies in one experiment.

MeSH Terms
Animals Cells, Cultured Chromatin/chemistry Chromatin Immunoprecipitation/methods DNA/analysis,isolation & purification Rats Reproducibility of Results Saccharomyces cerevisiae/genetics Time Factors
Chemicals
Chromatin DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nelson Joel D
Molecular and Cellular Biology Program, University of Washington, Seattle, WA 98109, USA.
Denisenko Oleg
Sova Pavel
Bomsztyk Karol
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gnj004
Published
2006-01-05
Epub
2006-00-05
Pages
e2
Language
English
Region
England
NLM ID
0411011
PMCID
PMC1325209
Subset
IM
Grants
NIDDK NIH HHS · R01 DK045978 · United States
NIDDK NIH HHS · DK45978 · United States
NIGMS NIH HHS · GM45134 · United States
NIDDK NIH HHS · R37 DK045978 · United States
NIGMS NIH HHS · R01 GM045134 · United States
External Links
PubMed source DOI: 10.1093/nar/gnj004 PMC Full Text View journal details
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