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PMID: 12853611 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Transient recruitment of the hnRNP K protein to inducibly transcribed gene loci.

Nucleic acids research ·Vol. 31 ·No. 14 ·2003-07-15 ·Pages 3954-62

Ostrowski J, Kawata Y, Schullery DS, Denisenko ON, Bomsztyk K

Abstract

The heterogeneous nuclear ribonucleoprotein K protein is an RNA- and DNA-binding protein implicated in the regulation of multiple processes that comprise gene expression. We used chromatin immunoprecipitation (ChIP) assays to explore K protein interactions with serum-inducible, constitutively expressed and untranscribed gene loci in vivo. In the rat HTC-IR hepatoma cell line, serum treatment induced transient increases in the mRNA levels of two immediate-early genes, egr-1 and c-myc. ChIP analysis showed that the induction of egr-1 and c-myc genes was associated with a transient recruitment of K protein to multiple sites within each of these loci, including the promoter and transcribed regions. In contrast, recruitment of K protein to the constitutively transcribed beta-actin locus and to randomly chosen non-transcribed loci was far weaker. In rat mesangial cells, c-myc was constitutively expressed while egr-1 remained serum responsive. In these cells, ChIP analysis showed serum-induced recruitment to the inducible egr-1 but not to the c-myc locus. Pre-treatment with the transcription inhibitor actinomycin D blocked the inducible but not the constitutive binding of K protein to these loci. Taken together, the results of this study suggest that the transient recruitment of K protein to serum-responsive loci depends on the inducible transcription of these immediate-early genes.

MeSH Terms
Animals Culture Media, Serum-Free/pharmacology DNA/metabolism DNA-Binding Proteins/genetics Early Growth Response Protein 1 Gene Expression Heterogeneous-Nuclear Ribonucleoprotein K/metabolism Immediate-Early Proteins/genetics Precipitin Tests/methods Protein Binding Proto-Oncogene Proteins c-myc/genetics RNA, Messenger/genetics,metabolism Reverse Transcriptase Polymerase Chain Reaction Transcription Factors/genetics Transcription, Genetic/drug effects,genetics Tumor Cells, Cultured
Chemicals
Culture Media, Serum-Free DNA-Binding Proteins Early Growth Response Protein 1 Egr1 protein, rat Heterogeneous-Nuclear Ribonucleoprotein K Immediate-Early Proteins Proto-Oncogene Proteins c-myc RNA, Messenger Transcription Factors DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ostrowski Jerzy
Department of Medicine, University of Washington, Seattle, WA 98195, USA.
Kawata Yasunobu
Schullery Daniel S
Denisenko Oleg N
Bomsztyk Karol
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkg452
Published
2003-07-15
Pages
3954-62
Language
English
Region
England
NLM ID
0411011
PMCID
PMC165967
Subset
IM
Grants
NIDDK NIH HHS · R01 DK045978 · United States
NIDDK NIH HHS · DK45978 · United States
FIC NIH HHS · TW05685 · United States
NIGMS NIH HHS · GM45134 · United States
NIDDK NIH HHS · R37 DK045978 · United States
NIGMS NIH HHS · R01 GM045134 · United States
FIC NIH HHS · R03 TW005685 · United States
External Links
PubMed source DOI: 10.1093/nar/gkg452 PMC Full Text View journal details
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