Home LiteratureArticle Details
PMID: 11470915 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Insulin alters heterogeneous nuclear ribonucleoprotein K protein binding to DNA and RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 98 ·No. 16 ·2001-07-31 ·Pages 9044-9

Ostrowski J, Kawata Y, Schullery DS, Denisenko ON, Higaki Y, Abrass CK, Bomsztyk K

Abstract

The interaction of the multimodular heterogeneous nuclear ribonucleoprotein (hnRNP) K protein with many of its protein and nucleic acid partners is regulated by extracellular signals. Acting as a docking platform, K protein could link signal-transduction pathways to DNA- and RNA-directed processes such as transcription, mRNA processing, transport, and translation. Treatment of hepatocyte culture with insulin increased K protein tyrosine phosphorylation. Insulin altered K protein interaction with RNA and DNA in vitro. Administration of insulin into mice had similar effects on K protein in liver. Coimmunoprecipitations of RNA with K protein revealed preferential in vivo K protein binding of a subset of transcripts, including the insulin-inducible c-fos mRNA. These results suggest a class of insulin pathways that signal nucleic acid-directed processes that involve K protein.

MeSH Terms
Animals Cell Line DNA/metabolism DNA-Binding Proteins/metabolism Heterogeneous-Nuclear Ribonucleoprotein K Heterogeneous-Nuclear Ribonucleoproteins Humans Insulin/pharmacology Mice Phosphorylation Protein Binding Proto-Oncogene Proteins c-fos/genetics RNA, Heterogeneous Nuclear/metabolism RNA, Messenger/genetics,metabolism RNA-Binding Proteins/metabolism Rats Ribonucleoproteins/metabolism
Chemicals
DNA-Binding Proteins Heterogeneous-Nuclear Ribonucleoprotein K Heterogeneous-Nuclear Ribonucleoproteins Insulin Proto-Oncogene Proteins c-fos RNA, Heterogeneous Nuclear RNA, Messenger RNA-Binding Proteins Ribonucleoproteins DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ostrowski J
Department of Medicine, University of Washington, Seattle, WA 98195, USA.
Kawata Y
Schullery D S
Denisenko O N
Higaki Y
Abrass C K
Bomsztyk K
References (30)
30 references, click to expand
  1. Role of tyrosine phosphorylation in the regulation of the interaction of heterogenous nuclear ribonucleoprotein K protein with its protein and RNA partners.
    J Biol Chem. 2000 Feb 4;275(5):3619-28 PMID: 10652358
  2. Regulation of GLUT1 gene transcription by the serine/threonine kinase Akt1.
    J Biol Chem. 1999 Jul 16;274(29):20281-6 PMID: 10400647
  3. Identification of new JNK substrate using ATP pocket mutant JNK and a corresponding ATP analogue.
    J Biol Chem. 2001 May 25;276(21):18090-5 PMID: 11259409
  4. Insulin action in cultured HTC and H35 rat hepatoma cells: receptor binding and hormone sensitivity.
    Endocrinology. 1981 Jan;108(1):44-51 PMID: 6161802
  5. Insulin rapidly stimulates tyrosine phosphorylation of a Mr-185,000 protein in intact cells.
    Nature. 1985 Nov 14-20;318(6042):183-6 PMID: 2414672
  6. Insulin as a growth factor in rat hepatoma cells. Stimulation of proto-oncogene expression.
    J Biol Chem. 1987 Aug 5;262(22):10893-7 PMID: 3301843
  7. Classification and purification of proteins of heterogeneous nuclear ribonucleoprotein particles by RNA-binding specificities.
    Mol Cell Biol. 1988 May;8(5):2237-41 PMID: 3386636
  8. Inhibition and stimulation of c-myc gene transcription by insulin in rat hepatoma cells. Insulin alters the intragenic pausing of c-myc transcription.
    J Biol Chem. 1991 Sep 25;266(27):17995-8001 PMID: 1917937
  9. Identification, molecular cloning, expression and chromosome mapping of a family of transformation upregulated hnRNP-K proteins derived by alternative splicing.
    J Mol Biol. 1994 Feb 11;236(1):33-48 PMID: 8107114
  10. Cloning and characterization of a single-stranded DNA binding protein that specifically recognizes deoxycytidine stretch.
    Nucleic Acids Res. 1994 Jan 11;22(1):53-8 PMID: 8127654
  11. Purification, cloning, and expression of a murine phosphoprotein that binds the kappa B motif in vitro identifies it as the homolog of the human heterogeneous nuclear ribonucleoprotein K protein. Description of a novel DNA-dependent phosphorylation process.
    J Biol Chem. 1994 Jul 1;269(26):17626-34 PMID: 8021272
  12. Association of the vav proto-oncogene product with poly(rC)-specific RNA-binding proteins.
    Mol Cell Biol. 1995 Mar;15(3):1324-32 PMID: 7862126
  13. Description of an IL-1-responsive kinase that phosphorylates the K protein. Enhancement of phosphorylation by selective DNA and RNA motifs.
    Biochemistry. 1995 Apr 25;34(16):5644-50 PMID: 7727425
  14. The K protein domain that recruits the interleukin 1-responsive K protein kinase lies adjacent to a cluster of c-Src and Vav SH3-binding sites. Implications that K protein acts as a docking platform.
    J Biol Chem. 1995 Nov 10;270(45):26976-85 PMID: 7592945
  15. Heterogeneous nuclear ribonucleoprotein K is a transcription factor.
    Mol Cell Biol. 1996 May;16(5):2350-60 PMID: 8628302
  16. Diverse signaling pathways in the cellular actions of insulin.
    Am J Physiol. 1996 Mar;270(3 Pt 1):E375-85 PMID: 8638681
  17. The Fyn tyrosine kinase binds Irs-1 and forms a distinct signaling complex during insulin stimulation.
    J Biol Chem. 1996 May 3;271(18):10583-7 PMID: 8631859
  18. Regulation of gene expression by insulin: analysis in single living cells.
    Biochem Soc Trans. 1996 May;24(2):378-84 PMID: 8736767
  19. Diverse molecular interactions of the hnRNP K protein.
    FEBS Lett. 1997 Feb 17;403(2):113-5 PMID: 9042948
  20. Insulin regulation of mitogen-activated protein kinase kinase (MEK), mitogen-activated protein kinase and casein kinase in the cell nucleus: a possible role in the regulation of gene expression.
    Biochem J. 1997 May 1;323 ( Pt 3):621-7 PMID: 9169593
  21. mRNA silencing in erythroid differentiation: hnRNP K and hnRNP E1 regulate 15-lipoxygenase translation from the 3' end.
    Cell. 1997 May 16;89(4):597-606 PMID: 9160751
  22. The product of the murine homolog of the Drosophila extra sex combs gene displays transcriptional repressor activity.
    Mol Cell Biol. 1997 Aug;17(8):4707-17 PMID: 9234727
  23. The insulin signalling system and the IRS proteins.
    Diabetologia. 1997 Jul;40 Suppl 2:S2-17 PMID: 9248696
  24. Molecular mechanisms for the control of translation by insulin.
    Biochem J. 1997 Dec 1;328 ( Pt 2):329-41 PMID: 9371685
  25. Identification of heterogeneous nuclear ribonucleoprotein K (hnRNP K) as a repressor of C/EBPbeta-mediated gene activation.
    J Biol Chem. 1998 Apr 24;273(17):10784-91 PMID: 9553145
  26. Differential effects of heterogeneous nuclear ribonucleoprotein K on Sp1- and Sp3-mediated transcriptional activation of a neuronal nicotinic acetylcholine receptor promoter.
    J Biol Chem. 1998 Jul 31;273(31):19877-83 PMID: 9677424
  27. Signaling mechanisms that regulate glucose transport.
    J Biol Chem. 1999 Jan 22;274(4):1865-8 PMID: 9890935
  28. Protein-protein interaction in insulin signaling and the molecular mechanisms of insulin resistance.
    J Clin Invest. 1999 Apr;103(7):931-43 PMID: 10194465
  29. Regulated interaction of protein kinase Cdelta with the heterogeneous nuclear ribonucleoprotein K protein.
    J Biol Chem. 1999 May 21;274(21):15101-9 PMID: 10329716
  30. Interaction of two multifunctional proteins. Heterogeneous nuclear ribonucleoprotein K and Y-box-binding protein.
    J Biol Chem. 2000 May 19;275(20):15498-503 PMID: 10809782
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.161284098
Published
2001-07-31
Epub
2001-00-24
Pages
9044-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC55370
Subset
IM
Grants
NIDDK NIH HHS · R01 DK045978 · United States
NIDDK NIH HHS · DK45978 · United States
NIGMS NIH HHS · GM45134 · United States
NIDDK NIH HHS · R37 DK045978 · United States
NIGMS NIH HHS · R01 GM045134 · United States
External Links
PubMed source DOI: 10.1073/pnas.161284098 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2