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PMID: 16362820 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Review

Organization of interphase chromatin.

Chromosoma ·Vol. 115 ·No. 1 ·2006-02-00 ·Pages 1-14

Horowitz-Scherer RA, Woodcock CL

Abstract

The organization of interphase chromatin spans many topics, ranging in scale from the molecular level to the whole nucleus, and its study requires a concomitant range of experimental approaches. In this review, we examine these approaches, the results they have generated, and the interfaces between them. The greatest challenge appears to be the integration of information on whole nuclei obtained by light microscopy with data on nucleosome-nucleosome interactions and chromatin higher-order structures, obtained in vitro using biophysical characterization, atomic force microscopy, and electron microscopy. We consider strategies that may assist in the integration process, and we review emerging technologies that promise to reduce the "resolution gap."

MeSH Terms
Animals Cell Nucleus/ultrastructure Chromatin/ultrastructure Humans Interphase Microscopy, Atomic Force Microscopy, Electron
Chemicals
Chromatin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Horowitz-Scherer Rachel A
Biology Department and Molecular and Cellular Biology Program, University of Massachusetts at Amherst, 01003, USA.
Woodcock Christopher L
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Article Info
Journal
Chromosoma
Abbr.
Chromosoma
ISSN
0009-5915
DOI
10.1007/s00412-005-0035-3
Published
2006-02-00
Epub
2005-00-17
Pages
1-14
Language
English
Region
Austria
NLM ID
2985138R
Subset
IM
Grants
NIGMS NIH HHS · GM070897 · United States
External Links
PubMed source DOI: 10.1007/s00412-005-0035-3 View journal details
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