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PMID: 15657424 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Common effects of acidic activators on large-scale chromatin structure and transcription.

Molecular and cellular biology ·Vol. 25 ·No. 3 ·2005-02-00 ·Pages 958-68

Carpenter AE, Memedula S, Plutz MJ, Belmont AS

Abstract

Large-scale chromatin decondensation has been observed after the targeting of certain acidic activators to heterochromatic chromatin domains. Acidic activators are often modular, with two or more separable transcriptional activation domains. Whether these smaller regions are sufficient for all functions of the activators has not been demonstrated. We adapted an inducible heterodimerization system to allow systematic dissection of the function of acidic activators, individual subdomains within these activators, and short acidic-hydrophobic peptide motifs within these subdomains. Here, we demonstrate that large-scale chromatin decondensation activity is a general property of acidic activators. Moreover, this activity maps to the same acidic activator subdomains and acidic-hydrophobic peptide motifs that are responsible for transcriptional activation. Two copies of a mutant peptide motif of VP16 (viral protein 16) possess large-scale chromatin decondensation activity but minimal transcriptional activity, and a synthetic acidic-hydrophobic peptide motif had large-scale chromatin decondensation activity comparable to the strongest full-length acidic activator but no transcriptional activity. Therefore, the general property of large-scale chromatin decondensation shared by most acidic activators is not simply a direct result of transcription per se but is most likely the result of the concerted action of coactivator proteins recruited by the activators' short acidic-hydrophobic peptide motifs.

MeSH Terms
Amino Acid Motifs/physiology Amino Acid Sequence Animals Antibiotics, Antineoplastic/toxicity CHO Cells Chromatin/metabolism Cricetinae Cricetulus Green Fluorescent Proteins/metabolism Herpes Simplex Virus Protein Vmw65/metabolism Molecular Sequence Data Sirolimus/toxicity Trans-Activators/metabolism Transcription, Genetic/drug effects,genetics
Chemicals
Antibiotics, Antineoplastic Chromatin Herpes Simplex Virus Protein Vmw65 Trans-Activators Green Fluorescent Proteins Sirolimus
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Carpenter Anne E
Department of Cell and Structural Biology, B107 CLSL, 601 S. Goodwin Avenue, Urbana, IL 61801, USA.
Memedula Sevinci
Plutz Matthew J
Belmont Andrew S
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
Published
2005-02-00
Pages
958-68
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC544008
Subset
IM
Grants
NIGMS NIH HHS · R01 GM042516 · United States
NIGMS NIH HHS · R01 GM058460 · United States
NIGMS NIH HHS · R01 GM 42516 · United States
NIGMS NIH HHS · R01 GM 58460 · United States
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