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PMID: 16325795 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Nucleoporins NPP-1, NPP-3, NPP-4, NPP-11 and NPP-13 are required for proper spindle orientation in C. elegans.

Developmental biology ·Vol. 289 ·No. 2 ·2006-01-15 ·Pages 360-71

Schetter A, Askjaer P, Piano F, Mattaj I, Kemphues K

Abstract

Nucleoporins are components of the nuclear pore, which is required for nucleo-cytoplasmic transport. We report a role for a subclass of nucleoporins in orienting the mitotic spindle in C. elegans embryos. RNAi-mediated depletion of any of five putative nucleoporins npp-1, npp-3, npp-4, npp-11, and npp-13 leads to indistinguishable spindle orientation defects. Transgenic worms expressing NPP-1::GFP or NPP-11::GFP show GFP localization at the nuclear envelope, consistent with their predicted function. NPP-1 interacts with the other nucleoporins in yeast two-hybrid assays, suggesting that the proteins affect spindle orientation by a common process. The failed orientation phenotype of npp-1(RNAi) is at least partially epistatic to the ectopic spindle rotation in the AB blastomere of par-3 mutant embryos. This suggests that NPP-1 contributes to the mechanics of spindle orientation. However, NPP-1 is also required for PAR-6 asymmetry at the two-cell stage, indicating that nucleoporins may be required to define cortical domains in the germ line blastomere P1. Nuclear envelope structure is abnormal in npp-1(RNAi) embryos, but the envelope maintains its integrity, and most nuclear proteins we assayed accumulate normally. These findings raise the possibility that these nucleoporins may have direct roles in orienting the mitotic spindle and the maintenance of cell polarity.

MeSH Terms
Adenosine Triphosphatases/genetics,metabolism Animals Caenorhabditis elegans/embryology,genetics,physiology Caenorhabditis elegans Proteins/genetics,metabolism,physiology Cell Differentiation/genetics Cell Division DNA, Recombinant/metabolism Dynactin Complex Fluorescent Antibody Technique Gene Expression Regulation, Developmental Membrane Proteins/genetics,metabolism Microscopy, Confocal Microtubule-Associated Proteins/genetics,metabolism Models, Genetic Nuclear Envelope/metabolism Nuclear Pore Complex Proteins/genetics,physiology RNA Interference Spindle Apparatus/physiology Two-Hybrid System Techniques
Chemicals
Caenorhabditis elegans Proteins DNA, Recombinant Dynactin Complex Membrane Proteins Microtubule-Associated Proteins NPP-3 protein, C elegans Nuclear Pore Complex Proteins ooc-3 protein, C elegans Adenosine Triphosphatases OOC-5 protein, C elegans
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schetter Aaron
Department of Molecular Biology and Genetics, Cornell University, 435 Biotechnology Building, Ithaca, NY 14853, USA.
Askjaer Peter
Piano Fabio
Mattaj Iain
Kemphues Kenneth
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Article Info
Journal
Developmental biology
Abbr.
Dev Biol
ISSN
0012-1606
DOI
10.1016/j.ydbio.2005.10.038
Published
2006-01-15
Epub
2005-00-02
Pages
360-71
Language
English
Region
United States
NLM ID
0372762
PMCID
PMC1405919
Subset
IM
Grants
NICHD NIH HHS · R01 HD027689 · United States
PHS HHS · 6M07617 · United States
NICHD NIH HHS · HD27698 · United States
External Links
PubMed source DOI: 10.1016/j.ydbio.2005.10.038 PMC Full Text View journal details
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