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PMID: 10982402 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

C. elegans nuclear envelope proteins emerin, MAN1, lamin, and nucleoporins reveal unique timing of nuclear envelope breakdown during mitosis.

Molecular biology of the cell ·Vol. 11 ·No. 9 ·2000-09-00 ·Pages 3089-99

Lee KK, Gruenbaum Y, Spann P, Liu J, Wilson KL

Abstract

Emerin, MAN1, and LAP2 are integral membrane proteins of the vertebrate nuclear envelope. They share a 43-residue N-terminal motif termed the LEM domain. We found three putative LEM domain genes in Caenorhabditis elegans, designated emr-1, lem-2, and lem-3. We analyzed emr-l, which encodes Ce-emerin, and lem-2, which encodes Ce-MAN1. Ce-emerin and Ce-MAN1 migrate on SDS-PAGE as 17- and 52-kDa proteins, respectively. Based on their biochemical extraction properties and immunolocalization, both Ce-emerin and Ce-MAN1 are integral membrane proteins localized at the nuclear envelope. We used antibodies against Ce-MAN1, Ce-emerin, nucleoporins, and Ce-lamin to determine the timing of nuclear envelope breakdown during mitosis in C. elegans. The C. elegans nuclear envelope disassembles very late compared with vertebrates and Drosophila. The nuclear membranes remained intact everywhere except near spindle poles during metaphase and early anaphase, fully disassembling only during mid-late anaphase. Disassembly of pore complexes, and to a lesser extent the lamina, depended on embryo age: pore complexes were absent during metaphase in >30-cell embryos but existed until anaphase in 2- to 24-cell embryos. Intranuclear mRNA splicing factors disassembled after prophase. The timing of nuclear disassembly in C. elegans is novel and may reflect its evolutionary position between unicellular and more complex eukaryotes.

MeSH Terms
Amino Acid Sequence Animals Caenorhabditis elegans/cytology,genetics,physiology Caenorhabditis elegans Proteins Cell Cycle/physiology DNA-Binding Proteins Embryo, Nonmammalian/physiology Humans Lamins Membrane Proteins/chemistry,genetics,metabolism Metaphase Mitosis/physiology Molecular Sequence Data Nuclear Envelope/ultrastructure Nuclear Pore/physiology,ultrastructure Nuclear Proteins/chemistry,genetics,metabolism Sequence Alignment Sequence Homology, Amino Acid Thymopoietins/chemistry,genetics,metabolism
Chemicals
Caenorhabditis elegans Proteins DNA-Binding Proteins LEMD3 protein, human Lamins Membrane Proteins Nuclear Proteins Thymopoietins emerin lem-2 protein, C elegans
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lee K K
Department of Cell Biology and Anatomy, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Gruenbaum Y
Spann P
Liu J
Wilson K L
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.11.9.3089
Published
2000-09-00
Pages
3089-99
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC14977
Subset
IM
Grants
NICHD NIH HHS · F32 HD008331 · United States
NIGMS NIH HHS · R01 GM048646 · United States
NIGMS NIH HHS · R01GM48646 · United States
NICHD NIH HHS · F32HD08331 · United States
External Links
PubMed source DOI: 10.1091/mbc.11.9.3089 PMC Full Text View journal details
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