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PMID: 1624447 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Physical mapping of repetitive extragenic palindromic sequences in Escherichia coli and phylogenetic distribution among Escherichia coli strains and other enteric bacteria.

Journal of bacteriology ·Vol. 174 ·No. 14 ·1992-07-00 ·Pages 4583-93

Dimri GP, Rudd KE, Morgan MK, Bayat H, Ames GF

Abstract

Repetitive extragenic palindromic (REP) sequences are highly conserved inverted repeat sequences originally discovered in Escherichia coli and Salmonella typhimurium. We have physically mapped these sequences in the E. coli genome by using Southern hybridization of an ordered phage bank of E. coli (Y. Kohara, K. Akiyama, and K. Isono, Cell 50:495-508, 1987) with generic REP probes derived from the REP consensus sequence. The set of REP probe-hybridizing clones was correlated with a set of clones expected to contain REP sequences on the basis of computer searches. We also show that a generic REP probe can be used in Southern hybridization to analyze genomic DNA digested with restriction enzymes to determine genetic relatedness among natural isolates of E. coli. A search for these sequences in other members of the family Enterobacteriaceae shows a consistent correlation between both the number of occurrences and the hybridization strength and genealogical relationship.

MeSH Terms

Base Sequence Chromosome Mapping/methods Enterobacteriaceae/genetics Escherichia coli/classification,genetics Genetic Variation Genome, Bacterial Molecular Sequence Data Phylogeny Repetitive Sequences, Nucleic Acid/genetics Species Specificity

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Dimri G P

Department of Molecular and Cell Biology, University of California, Berkeley 94720.

Rudd K E

Morgan M K

Bayat H

Ames G F

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.174.14.4583-4593.1992

Published

1992-07-00

Pages

4583-93

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC206253

Subset

Grants

NIGMS NIH HHS · 1-F32 GM12156 · United States

NIGMS NIH HHS · GM39415 · United States

External Links

PubMed source DOI: 10.1128/jb.174.14.4583-4593.1992 PMC Full Text View journal details

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Physical mapping of repetitive extragenic palindromic sequences in Escherichia coli and phylogenetic distribution among Escherichia coli strains and other enteric bacteria.

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