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PMID: 2178927 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Tandem chromosomal duplications: role of REP sequences in the recombination event at the join-point.

The EMBO journal ·Vol. 9 ·No. 3 ·1990-03-00 ·Pages 939-46

Shyamala V, Schneider E, Ames GF

Abstract

We show that a family of prokaryotic repetitive sequences, called REP (repetitive extragenic palindromic), (Stern et al., 1984) is involved in the formation of chromosomal rearrangements such as duplications. The join-points of seven RecA+ tandem duplications previously characterized in Salmonella typhimurium, that fuse the hisD gene to distant foreign promoters, were cloned and sequenced. In all seven cases they are shown to have originated by recombination between distant REP sequences. Importantly, several join-points had also occurred at REP sequences even in a RecA-background. Thus, REPs can recombine with each other by a RecA(-)-independent mechanism involved in the generation of chromosomal rearrangements. While all RecA+ duplications analysed resulted from recombination between REP sequences, some RecA-duplications did occur also outside of REP sequences, in one case by recombination within a 7 bp homology. Possible roles for the known interaction between DNA gyrase and REP in chromosomal rearrangements are discussed.

MeSH Terms
Base Sequence Chromosomes, Bacterial Cloning, Molecular Genes, Bacterial Molecular Sequence Data Oligonucleotide Probes Polymerase Chain Reaction Promoter Regions, Genetic Rec A Recombinases/genetics Recombination, Genetic Repetitive Sequences, Nucleic Acid Restriction Mapping Salmonella typhimurium/genetics
Chemicals
Oligonucleotide Probes Rec A Recombinases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shyamala V
Division of Biochemistry and Molecular Biology, University of California, Berkeley 94720.
Schneider E
Ames G F
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-03-00
Pages
939-46
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551756
Subset
IM
Grants
NIDDK NIH HHS · DK12121 · United States
External Links
PubMed source PMC Full Text View journal details
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