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PMID: 16227580 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pairing between gypsy insulators facilitates the enhancer action in trans throughout the Drosophila genome.

Molecular and cellular biology ·Vol. 25 ·No. 21 ·2005-11-00 ·Pages 9283-91

Kravchenko E, Savitskaya E, Kravchuk O, Parshikov A, Georgiev P, Savitsky M

Abstract

The Suppressor of the Hairy wing [Su(Hw)] binding region within the gypsy retrotransposon is the best known chromatin insulator in Drosophila melanogaster. According to previous data, two copies of the gypsy insulator inserted between an enhancer and a promoter neutralize each other's actions, which is indicative of an interaction between the protein complexes bound to the insulators. We have investigated the role of pairing between the gypsy insulators located on homologous chromosomes in trans interaction between yellow enhancers and a promoter. It has been shown that trans activation of the yellow promoter strongly depends on the site of the transposon insertion, which is evidence for a role of surrounding chromatin in homologous pairing. The presence of the gypsy insulators in both homologous chromosomes even at a distance of 9 kb downstream from the promoter dramatically improves the trans activation of yellow. Moreover, the gypsy insulators have proved to stabilize trans activation between distantly located enhancers and a promoter. These data suggest that gypsy insulator pairing is involved in communication between loci in the Drosophila genome.

MeSH Terms
Animals Chromatin/genetics Drosophila Proteins/genetics Drosophila melanogaster/genetics Enhancer Elements, Genetic Genome, Insect Promoter Regions, Genetic Retroelements/genetics Transcriptional Activation
Chemicals
Chromatin Drosophila Proteins Retroelements y protein, Drosophila
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kravchenko Elena
Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., 119334 Moscow, Russia.
Savitskaya Ekaterina
Kravchuk Oksana
Parshikov Alexander
Georgiev Pavel
Savitsky Mikhail
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.25.21.9283-9291.2005
Published
2005-11-00
Pages
9283-91
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1265844
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.25.21.9283-9291.2005 PMC Full Text View journal details
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