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PMID: 9990850 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Core promoter elements can regulate transcription on a separate chromosome in trans.

Genes & development ·Vol. 13 ·No. 3 ·1999-02-01 ·Pages 253-8

Morris JR, Geyer PK, Wu CT

Abstract

Transvection can cause the expression of a gene to be sensitive to the proximity of a homolog. It can account for many cases of intragenic complementation at the Drosophila yellow gene, where one mode of transvection involves the action of enhancers in trans on a promoter present on a separate chromosome. Our goal was to identify cis-acting elements that regulate the trans action of enhancers. Using gene replacement, we altered two core promoter elements at yellow and tested the resulting alleles for their ability to support transvection. We found that the TATA box and initiator element can regulate transvection.

MeSH Terms
Drosophila Proteins Gene Expression Regulation Insect Proteins/genetics Promoter Regions, Genetic TATA Box Transcription, Genetic
Chemicals
Drosophila Proteins Insect Proteins y protein, Drosophila
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Morris J R
Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115 USA.
Geyer P K
Wu C T
References (36)
36 references, click to expand
  1. Altered gene expression in plants due to trans interactions between homologous genes.
    Trends Biotechnol. 1990 Dec;8(12):340-4 PMID: 1366894
  2. Trans regulation in the Ultrabithorax gene of Drosophila: alterations in the promoter enhance transvection.
    EMBO J. 1992 Oct;11(10):3645-52 PMID: 1396564
  3. Molecular analysis of the yellow locus of Drosophila.
    EMBO J. 1986 Dec 20;5(13):3597-605 PMID: 16052739
  4. Tissue-specific transcriptional enhancers may act in trans on the gene located in the homologous chromosome: the molecular basis of transvection in Drosophila.
    EMBO J. 1990 Jul;9(7):2247-56 PMID: 2162766
  5. Genetic evidence for promoter competition in Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Nov;8(11):4608-15 PMID: 2850465
  6. On the molecular mechanism of gypsy-induced mutations at the yellow locus of Drosophila melanogaster.
    EMBO J. 1986 Oct;5(10):2657-62 PMID: 3096713
  7. A genetic analysis of the Suppressor 2 of zeste complex of Drosophila melanogaster.
    Genetics. 1995 May;140(1):139-81 PMID: 7635282
  8. Modulation of enhancer-promoter interactions by insulators in the Drosophila embryo.
    Nature. 1995 Aug 10;376(6540):533-6 PMID: 7637789
  9. TAFs and TFIIA mediate differential utilization of the tandem Adh promoters.
    Cell. 1995 Aug 25;82(4):565-75 PMID: 7664336
  10. Transvection in the iab-5,6,7 region of the bithorax complex of Drosophila: homology independent interactions in trans.
    Genetics. 1995 Feb;139(2):815-33 PMID: 7713434
  11. Cis and trans interactions between the iab regulatory regions and abdominal-A and abdominal-B in Drosophila melanogaster.
    Genetics. 1995 Feb;139(2):835-48 PMID: 7713435
  12. Promoter elements in Drosophila melanogaster revealed by sequence analysis.
    Genetics. 1995 Mar;139(3):1359-69 PMID: 7768444
  13. Targeted transposition at the vestigial locus of Drosophila melanogaster.
    Genetics. 1994 Dec;138(4):1127-35 PMID: 7896095
  14. Compatibility between enhancers and promoters determines the transcriptional specificity of gooseberry and gooseberry neuro in the Drosophila embryo.
    EMBO J. 1994 Jan 15;13(2):400-6 PMID: 8313885
  15. Gene targeting of a plasmid-borne sequence to a double-strand DNA break in Drosophila melanogaster.
    Mol Cell Biol. 1996 Feb;16(2):522-8 PMID: 8552079
  16. Effects of the su(Hw) insulator protein on the expression of the divergently transcribed Drosophila yolk protein genes.
    EMBO J. 1995 Dec 15;14(24):6258-67 PMID: 8557045
  17. Reduction of transcription by homologue asynapsis in Drosophila imaginal discs.
    Nature. 1996 Jun 27;381(6585):807-10 PMID: 8657287
  18. Promoter specificity mediates the independent regulation of neighboring genes.
    Genes Dev. 1996 May 15;10(10):1260-70 PMID: 8675012
  19. Genetic and molecular analysis of the gypsy chromatin insulator of Drosophila.
    Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9378-83 PMID: 8790337
  20. Meiotic recombination hotspots.
    Annu Rev Genet. 1995;29:423-44 PMID: 8825482
  21. One-tube direct PCR from whole Drosophila melanogaster adults.
    Trends Genet. 1996 Nov;12(11):457 PMID: 8973152
  22. Interactions of Drosophila Ultrabithorax regulatory regions with native and foreign promoters.
    Genetics. 1997 Jan;145(1):123-37 PMID: 9017395
  23. The role of insulator elements in defining domains of gene expression.
    Curr Opin Genet Dev. 1997 Apr;7(2):242-8 PMID: 9115431
  24. The transcriptional basis of chromosome pairing.
    J Cell Sci. 1997 May;110 ( Pt 9):1033-40 PMID: 9175699
  25. Pairing sites and the role of chromosome pairing in meiosis and spermatogenesis in male Drosophila.
    Curr Top Dev Biol. 1998;37:77-115 PMID: 9352184
  26. Mechanism of synergy between TATA and initiator: synergistic binding of TFIID following a putative TFIIA-induced isomerization.
    Genes Dev. 1997 Nov 15;11(22):3007-19 PMID: 9367983
  27. Different core promoters possess distinct regulatory activities in the Drosophila embryo.
    Genes Dev. 1998 Feb 15;12(4):547-56 PMID: 9472023
  28. Competitive edge at the imprinted Prader-Willi/Angelman region?
    Nat Genet. 1998 Mar;18(3):206-8 PMID: 9500535
  29. Selectivity, sharing and competitive interactions in the regulation of Hoxb genes.
    EMBO J. 1998 Mar 16;17(6):1788-98 PMID: 9501100
  30. Trans-sensing effects: the ups and downs of being together.
    Cell. 1998 May 1;93(3):329-32 PMID: 9590167
  31. Transvection in the Drosophila Abd-B domain: extensive upstream sequences are involved in anchoring distant cis-regulatory regions to the promoter.
    Genetics. 1998 Jun;149(2):1031-50 PMID: 9611211
  32. The effect of distance on long-range chromatin interactions.
    Mol Cell. 1997 Dec;1(1):131-9 PMID: 9659910
  33. The scs and scs' insulator elements impart a cis requirement on enhancer-promoter interactions.
    Mol Cell. 1998 Jan;1(2):301-8 PMID: 9659926
  34. Going the distance: a current view of enhancer action.
    Science. 1998 Jul 3;281(5373):60-3 PMID: 9679020
  35. Two modes of transvection: enhancer action in trans and bypass of a chromatin insulator in cis.
    Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10740-5 PMID: 9724774
  36. An analysis of transvection at the yellow locus of Drosophila melanogaster.
    Genetics. 1999 Feb;151(2):633-51 PMID: 9927457
Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.13.3.253
Published
1999-02-01
Pages
253-8
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC316431
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.13.3.253 PMC Full Text View journal details
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